NUDT6, the FGF-2’s antisense gene, showed associations with fat deposition related traits in pigs

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• 作者：Ling Sun (1)
  Shaobo Yu (1)
  Hongyang Wang (1)
  Bin Fan (1)
  Bang Liu (1) liubang@mail.hzau.edu.cn
• 关键词：NUDT6 – Variant transcript – FGF ; 2 – Express profile – Association analysis – Pig
• 刊名：Molecular Biology Reports
• 出版年：2012
• 出版时间：April 2012
• 年：2012
• 卷：39
• 期：4
• 页码：4119-4126
• 全文大小：384.6 KB
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• 作者单位：1. Lab of Molecular Biology and Animal Breeding, Key Laboratory of Agricultural Animal Genetics, Breeding, and Reproduction of Ministry of Education, Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture, Huazhong Agricultural University, Wuhan, 430070 People’s Republic of China
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Animal Anatomy, Morphology and Histology
  Animal Biochemistry
  
• 出版者：Springer Netherlands
• ISSN：1573-4978

文摘

The nudix (nucleoside diphosphate linked moiety X)-type motif 6 (Nudt6) belongs to the cytosolic Nudix hydrolase family genes, and it has been identified as a fibroblast growth factor 2 (Fgf-2)’s anti-sense gene in humans and rats, and can regulate Fgf-2 stability by its 3′ un-translated region (UTR) of mRNA. Here we cloned two completed mRNA of porcine NUDT6 variant transcripts and then analyzed the overlapping relationships of 3′UTR ends between NUDT6 and FGF-2 genes. The expression patterns of NUDT6 were detected in heart, liver, spleen, lung, kidney, muscle and fat tissues, suggesting a widespread mRNA expression of this gene in pigs. The real-time PCR results revealed the reverse expression patterns of NUDT6 and FGF-2 in Tongcheng and Yorkshire pig’s tissues including heart, live, spleen, lung, kidney and fat tissues. A C/T single nucleotide polymorphism in NUDT6 was genotyped in two experimental populations, and the association analyses revealed that it was significantly associated with intramuscular fat percentage (P < 0.01) and intramuscular lipid (P < 0.05) in experiment population 1, and it was significantly associated with percentage of leaf and caul fat (P < 0.05), percentage of leaf fat (P < 0.05), proportion of lean and bone of the ham (P < 0.05) in experimental population 2. The findings from this study could offer new evidences that NUDT6 maybe a candidate gene for fat deposition in pigs.