Identification of S-alleles in almond using multiplex PCR
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- 作者：Sá ; nchez Pé ; rez ; Raquel ; Dicenta ; Federico ; Martí ; nez Gó ; mez ; Pedro
- 关键词：almond ; breeding program ; marker ; assisted selection ; molecular markers ; PCR ; Prunus ; self ; incompatibility
- 刊名：Euphytica
- 出版年：2004
- 出版时间：2004
- 年：2004
- 卷：138
- 期：3
- 页码：263-269
- 全文大小：406 KB
- 刊物类别：Biomedical and Life Sciences
- 刊物主题：Life Sciences
Plant Physiology
Plant Sciences
Ecology - 出版者：Springer Netherlands
- ISSN：1573-5060
文摘
The S -genotypes of eight almond (Prunus dulcis Miller (D.A. Webb)) cultivars from different geographical origins and of nine new selections from the CEBAS-CSIC (Murcia, Spain) breeding program were determined using single and multiplex PCR with different sets of specific oligonucleotide primers. The results of PCR using the AS1II- and AmyC5R-specific primers showed amplification in a single reaction of 10 different self-incompatibility alleles and of the self-compatibility allele Sf . However, the amplified fragments of the Sf allele were of similar sizes to those amplified from the S 3 self-incompatibility allele. For this reason, a specific PCR primer CEBASf was designed from the intron sequence of Sf . A multiplex-PCR reaction using the AS1II, CEBASf and AmyC5R primers permitted unequivocal identification of the 10 self-incompatibility alleles and of the self-compatibility allele. Multiplex PCR opens the possibility to identify new S -alleles using different sets of primers. The applications of these PCR markers in the almond-breeding programs are discussed.