Preparation of hydrolysates from tobacco stalks and ethanolic fermentation by Saccharomyces cerevisiae
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- 作者：Martí ; n ; Carlos ; Ferná ; ndez ; Teresa ; Garcí ; a ; Ramó ; n ; Carrillo ; Eugenio ; Marcet ; Marcelo ; Galbe ; Mats ; Jö ; nsson ; Leif J.
- 关键词：Ethanol ; fermentation inhibitors ; lignocellulose hydrolysates ; Saccharomyces cerevisiae ; tobacco stalks
- 刊名：World Journal of Microbiology and Biotechnology
- 出版年：2002
- 出版时间：2002
- 年：2002
- 卷：18
- 期：9
- 页码：857-862
- 全文大小：109 KB
- 刊物类别：Chemistry and Materials Science
- 刊物主题：Chemistry
Applied Microbiology
Biotechnology
Biochemistry
Environmental Biotechnology
Microbiology - 出版者：Springer Netherlands
- ISSN：1573-0972
文摘
Chipped tobacco stalks were subjected to steam pretreatment at 205 °C for either 5 or 10 min before enzymatic hydrolysis. Glucose (15.4–17.1 g/l) and xylose (4.5–5.0 g/l) were the most abundant monosaccharides in the hydrolysates. Mannose, galactose and arabinose were also detected. The hydrolysate produced by pretreatment for 10 min contained higher levels of all sugars than the 5 min‐pretreated hydrolysate. The amounts of inhibitory compounds found in the hydrolysates were relatively low and increased with increasing pretreatment time. The hydrolysates were fermented with baker's yeast. Ethanol yield, maximum volumetric productivity and specific productivity were used as criteria of fermentability of the hydrolysates. The fermentation of the hydrolysates was only slightly inhibited compared to reference solutions having a similar composition of fermentable sugars. The ethanol yield in the hydrolysates was 0.38–0.39 g/g of initial fermentable sugars, whereas it was 0.42 g/g in the reference. The biomass yield was twofold lower in the hydrolysates than in the reference. The fermentation inhibition caused by the tobacco stalk hydrolysates was less than that caused by sugarcane bagasse hydrolysates obtained under the same hydrolysis conditions.