Respiratory stimulus in Rhizobium sp. by legume lectins

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• 作者：Mart&iacute ; nez ; Cosme R. ; Albertini ; Alessandro V.P. ; Figueiredo ; Má ; rcia V.B. ; Silva ; Valdinete L. ; Sampaio ; Alexandre H. ; Cavada ; Benildo S. ; Lima&hyphen ; Filho ; José ; L.
• 关键词：Canavalia brasiliensis ; Cratylia floribunda ; lectin ; Phaseolus vulgaris ; respiration ; Rhizobium etli ; Rhizobium tropici ; Vatairea macrocarpa
• 刊名：World Journal of Microbiology and Biotechnology
• 出版年：2004
• 出版时间：2004
• 年：2004
• 卷：20
• 期：1
• 页码：77-83
• 全文大小：449 KB
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Applied Microbiology
  Biotechnology
  Biochemistry
  Environmental Biotechnology
  Microbiology
  
• 出版者：Springer Netherlands
• ISSN：1573-0972

文摘

High molecular weight lectins (> 100 kDa) from seeds of the legumes Canavalia brasiliensis (CnBr), Cratylia floribunda (CFL), Phaseolus vulgaris (PHA) and Vatairea macrocarpa (VML), temporarily stimulate the respiration of Rhizobium tropici‐CIAT899 and R. etli‐CFN42. These stimulants were significant (P < 0.05) in bacterial suspensions (> 2.85 mg dry biomass ml⁻¹), having at least 6200 molecules of lectins per bacteria. The VML (20 &mgr;g ml⁻¹), induced specific O₂ demand of 2.3–2.5 ηM O₂ min⁻¹ mg dry biomass⁻¹, in CFN42 and CIAT899, respectively. However, CnBr, CFL and PHA induced smaller demands of O₂ (5×), in both strains. The order of affinities of the lectins was approximately VML > PHA > CFL > CnBr, with regard to respiratory stimuli in CIAT899 strain. The co‐administration of 10 &mgr;g VML ml⁻¹ and 9.8 ηM galactose, in CIAT899 suspensions, reduced the respiratory stimuli significantly in relation to the treatment with VML alone. These respiratory stimuli, induced by the lectins, increase the significance of the interaction lectin × Rhizobium in terms of bacterial physiology. Its understanding could be important in relation to bacterial symbiotic behaviour.