Microscopic and UPLC–UV–MS analyses of authentic and commercial yohimbe (Pausinystalia johimbe) bark samples

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• 作者：Vijayasankar Raman (1)
  Bharathi Avula (1)
  Ahmed M. Galal (1)
  Yan-Hong Wang (1)
  Ikhlas A. Khan (1) (2) (3)
  
• 关键词：Pausinystalia johimbe ; Yohimbe and yohimbine ; Light and scanning electron microscopy ; UPLC–UV–MS ; Authentication
• 刊名：Journal of Natural Medicines
• 出版年：2013
• 出版时间：January 2013
• 年：2013
• 卷：67
• 期：1
• 页码：42-50
• 全文大小：2531KB
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• 作者单位：Vijayasankar Raman (1)
  Bharathi Avula (1)
  Ahmed M. Galal (1)
  Yan-Hong Wang (1)
  Ikhlas A. Khan (1) (2) (3)
  
  1. National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, School of Pharmacy, University of Mississippi, Oxford, MS, 38677, USA
  2. Department of Pharmacognosy, Research Institute of Pharmaceutical Sciences, School of Pharmacy, University of Mississippi, Oxford, MS, 38677, USA
  3. Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia
  
• ISSN：1861-0293

文摘

Yohimbine is the major alkaloid found in the stem bark of yohimbe, Pausinystalia johimbe (Rubiaceae), an evergreen tree native to Africa. The objectives of the current study were to provide a detailed anatomy of yohimbe bark, as well as to determine the quantity of yohimbine in the raw yohimbe products sold online. Twelve commercial raw materials of yohimbe were analyzed by microscopic and ultra performance liquid chromatography–UV–MS methods. The study revealed that three samples were probably adulterated and four other samples contained various levels of impurities. Yohimbine was not detected in one sample, whereas its presence in other samples was found to be in the range 0.1-.91%. The present work also provides a detailed anatomy of the stem bark of yohimbe, with light and scanning electron microscopy images, for proper identification and authentication.