Molecular imaging analysis of Rab GTPases in the regulation of phagocytosis and macropinocytosis

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• 作者：Youhei Egami
• 关键词：Live ; cell imaging ; Macropinocytosis ; Membrane trafficking ; Phagocytosis ; Rab GTPase
• 刊名：Anatomical Science International
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：91
• 期：1
• 页码：35-42
• 全文大小：1,105 KB
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• 作者单位：Youhei Egami (1)
  
  1. Department of Histology and Cell Biology, School of Medicine, Kagawa University, Miki, Kagawa, 761-0793, Japan
  
• 刊物主题：Anatomy; Human Physiology; Animal Physiology; Cell Biology; Animal Anatomy / Morphology / Histology;
• 出版者：Springer Japan
• ISSN：1447-073X

文摘

Phagocytosis and macropinocytosis, actin-dependent endocytic pathways that mediate the uptake of particles and fluid, respectively, are fundamental routes that enable cells to sample their environment, eliminate pathogens and endogenous cell debris, and contribute to immunoprotection and the maintenance of tissue homeostasis. These processes require a well-organized network of actin cytoskeletal remodeling and membrane transport, which are spatiotemporally regulated by small GTPases. The Rab family of small GTPases, which functions as molecular switches, plays central roles in intracellular membrane trafficking. Although multiple Rab proteins are localized to phagosomes and regulate phagosome maturation, the precise role of each Rab family member in Fc纬 receptor (Fc纬R)-mediated phagocytosis is not fully characterized. Recently, we revealed that Rab35 and Rab20 are important regulators of phagosome formation and maturation, respectively. This review summarizes the functional implication of these Rab GTPases during Fc纬R-mediated phagocytosis in macrophages. Currently, compared with our knowledge of the regulatory mechanisms of receptor-mediated endocytosis including phagocytosis, the molecular components and signaling cascades of macropinocytosis remain poorly elucidated. Our time-lapse imaging showed that several Rab GTPases are sequentially recruited to the membrane of macropinosomes. Based on our observations, these findings regarding the spatiotemporal localization of Rab GTPases during macropinocytosis are introduced. Keywords Live-cell imaging Macropinocytosis Membrane trafficking Phagocytosis Rab GTPase