Rapid drug susceptibility test of Mycobacterium tuberculosis using microscopic time-lapse imaging in an agarose matrix

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• 作者：Jungil Choi ; Jungheon Yoo ; Ki-jung Kim…
• 关键词：Tuberculosis ; Drug susceptibility test ; Microscopic imaging ; Automation
• 刊名：Applied Microbiology and Biotechnology
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：100
• 期：5
• 页码：2355-2365
• 全文大小：1,519 KB
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• 作者单位：Jungil Choi (1) (2)
  Jungheon Yoo (2)
  Ki-jung Kim (3)
  Eun-Geun Kim (2)
  Kyung Ock Park (2)
  Hyejin Kim (4)
  Haeun Kim (4)
  Hyunju Jung (4)
  Taeyoung Kim (5)
  Myungjin Choi (5)
  Hee Chan Kim (3) (6)
  Sungweon Ryoo (4)
  Yong-Gyun Jung (2)
  Sunghoon Kwon (1) (2)
  
  1. Department of Electrical Engineering and Computer Science, Seoul National University, San 56-1, Daehak-dong, Gwanak-gu, Seoul, 151-744, Republic of Korea
  2. QuantaMatrix Inc., 104-213, Seoul National University, Seoul, Republic of Korea
  3. Department of Biomedical Engineering, Seoul National University, 28 Yongon-dong, Chongno-gu, Seoul, Republic of Korea
  4. Korean Institute of Tuberculosis, Mansu-ri 482, Osong, Cheongju, Chungcheongbuk-do, 361-954, Republic of Korea
  5. InSpace Co., Ltd., 96, Gajeongbuk-ro, Yuseong-gu, Daejeon, 305-343, Republic of Korea
  6. Department of Biomedical Engineering, Medical Research Center, Seoul National University College of Medicine, Seoul, Korea
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Biotechnology
  Microbiology
  Microbial Genetics and Genomics
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-0614

文摘

Tuberculosis (TB) is a major global health problem, and multi-drug-resistant TB (MDR-TB) and extensively drug-resistant TB (XDR-TB) are spreading throughout the world. However, conventional drug susceptibility test (DST) methods, which rely on the detection of the colony formation on a solid medium, require 1–2 months to the result. A rapid and accurate DST is necessary to identify patients with drug-resistant TB and treat them with appropriate drugs. Here, we used microscopic imaging of Mycobacterium tuberculosis (MTB) immobilized in an agarose matrix for a rapid DST. The agarose matrix, which was molded in a microfluidic chip, was inoculated with MTB, and TB drugs in liquid culture medium diffused throughout the agarose to reach the MTB immobilized in the agarose matrix. After the responses of MTB to drugs were tracked with an automated microscopic system, an image-processing program automatically determined the susceptibility and resistance of MTB to specific doses of TB drugs. The automatic DST system was able to assess the drug susceptibility of various drug-resistant clinical TB strains within 9 days with an accuracy comparable to that of conventional method. Our rapid DST method based on microscopic time-lapse imaging greatly reduces the time required for a DST and can be used to rapidly and accurately treat TB patients.