Long-term time-lapse live imaging reveals extensive cell migration during annelid regeneration
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- 作者：Eduardo E. Zattara ; Kate W. Turlington ; Alexandra E. Bely
- 关键词：Annelid neoblast ; Cell migration ; Developmental dynamics ; Growth rates ; In ; vivo studies ; Regeneration ; Time ; lapse imaging
- 刊名：BMC Developmental Biology
- 出版年：2016
- 出版时间：December 2016
- 年：2016
- 卷：16
- 期：1
- 全文大小：2,676 KB
- 刊物主题：Developmental Biology; Animal Models; Life Sciences, general;
- 出版者：BioMed Central
- ISSN：1471-213X
文摘
Background Time-lapse imaging has proven highly valuable for studying development, yielding data of much finer resolution than traditional “still-shot” studies and allowing direct examination of tissue and cell dynamics. A major challenge for time-lapse imaging of animals is keeping specimens immobile yet healthy for extended periods of time. Although this is often feasible for embryos, the difficulty of immobilizing typically motile juvenile and adult stages remains a persistent obstacle to time-lapse imaging of post-embryonic development.