A New Stationary Phase for Analysis of Hydrophobic Compounds by RP-LC

详细信息    查看全文

• 作者：Giselle O. Carvalho ; Carla G. A. da Silva ; Anizio M. Faria
• 关键词：Column liquid chromatography ; Stationary phase ; Poly(dimethyl ; co ; alkylmethylsiloxane) ; Hydrophobic interaction ; Microwave radiation ; Immobilized phases
• 刊名：Chromatographia
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：79
• 期：1-2
• 页码：19-27
• 全文大小：942 KB
• 参考文献：1.Kirkland J (2004) J Chromatogr A 1060:9–21CrossRef
  2.Tanaka N, Nagayama H, Kobayashi H, Ikegami T, Hosoya K, Ishizuka N, Minakuchi H, Nakanishi K, Cabrera K, Lubda D (2000) J High Resolut Chromatogr 23:111–116CrossRef
  3.Qiu HD, Liang XJ, Sun M, Jiang SX (2011) Anal Bioanal Chem 399:3307–3322CrossRef
  4.Fekete S, Oláh E, Fekete J (2012) J Chromatogr A 1228:57–71CrossRef
  5.Liang XJ, Wang S, Liu SJ, Liu X, Jiang SX (2012) J Sep Sci 35:2003–2009CrossRef
  6.Schure MR, Rafferty JL, Zhang L, Siepmann JI (2013) LC-GC N Am 31:630–637
  7.Löchmuller CH, Wilder DR (1979) J Chromatogr Sci 17:574–579CrossRef
  8.Sándi Á, Bede Á, Szepesy L, Rippel G (1997) Chromatographia 45:206–214CrossRef
  9.Strohschein S, Pursch M, Lubda D, Albert K (1988) Anal Chem 70:13–18CrossRef
  10.Buszewski B, Kowalska S, Stepnowski P (2006) J Sep Sci 29:1116–1125CrossRef
  11.Tonhi E, Collins KE, Collins CH (2006) J Chromatogr A 1119:135–139CrossRef
  12.Leo AJ (1987) J Pharmacol Sci 76:166–168CrossRef
  13.Hayward SJ, Lei YD, Wania F (2006) Environ Toxicol Chem 25:2018–2027CrossRef
  14.Faria AM, Collins CH, Jardim ICSF (2009) J Braz Chem Soc 20:1385–1398CrossRef
  15.Begnini FR, Jardim ICSF (2013) J Chromatogr A 1297:113–122CrossRef
  16.da Silva CGA, Collins CH, Lesellier E, West C (2013) J Chromatogr A 1315:176–187CrossRef
  17.Dib SR, Faria AM (2014) Chromatographia 77:533–541CrossRef
  18.Silva RB, Gushikem Y, Collins CH (2001) J Sep Sci 24:49–54CrossRef
  19.Faria AM, Collins KE, Collins CH (2007) J Chromatogr A 1156:51–59CrossRef
  20.Borges EM, Collins CH (2012) J Chromatogr A 1227:174–180CrossRef
  21.Lopes NP, Collins KE, Jardim ICSF (2004) J Chromatogr A 1030:225–229CrossRef
  22.Morais LSR, Jardim ICSF (2005) J Chromatogr A 1073:127–135CrossRef
  23.Collins CH, Silva CR, Faria AM, Collins KE, Jardim ICSF (2009) J Braz Chem Soc 20:604–612CrossRef
  24.Novais AS, Ribeiro Filho JF, Amaral EMF, Faria AM (2015) Quim Nova 38:274–279. doi:10.​5935/​0100-4042.​20140312
  25.Kimata K, Iwaguchi K, Onishi S, Jinno K, Eksteen R, Hosoya K, Arki M, Tanaka N (1989) J Chromatogr Sci 27:721–728CrossRef
  26.Bachmann S, Melo LFC, Silva RB, Anazawa TA, Jardim ICSF, Collins KE, Collins CH, Albert K (2001) Chem Mater 13:1874–1879CrossRef
  
• 作者单位：Giselle O. Carvalho (1)
  Carla G. A. da Silva (2)
  Anizio M. Faria (1)
  
  1. Química, Faculdade de Ciências Integradas do Pontal, Universidade Federal de Uberlândia, Ituiutaba, MG, 38304-402, Brazil
  2. Instituto de Química, Universidade Estadual de Campinas, Campinas, SP, 13083-970, Brazil
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Analytical Chemistry
  Organic Chemistry
  Pharmacy
  Biochemistry
  Plant Sciences
  Measurement Science and Instrumentation
  
• 出版者：Vieweg Verlag
• ISSN：1612-1112

文摘

Hydrophobic selectivity is a key factor that has made C₁₈-bonded phases the main separation materials for HPLC since its development. However, hydrophobic compounds tend to be strongly retained in these phases meaning that large amounts of organic mobile phases are required. In this work, a new stationary phase was prepared from the immobilization of poly(dimethyl-co-alkylmethylsiloxane) (PDAS) over silica particles using microwave radiation. The optimized conditions for preparation of the stationary phase used 720 W of radiation for 10 min. The chromatographic performance and methylene selectivity in the separation of hydrophobic compounds by this new Si(PDAS) phase were similar to those obtained by typical C₁₈-bonded phases. However, the lower hydrophobicity of the organic layer in the Si(PDAS) phase resulted in separation about 40 % faster than typical C₁₈ phases, requiring more aqueous mobile phases. The Si(PDAS) phase showed lower silanophilic activity than that of typical C₁₈ phases due to a more effective polymeric coating of the silica surface.