An electrochemical immunosensor for anti-T. cruzi IgM antibodies, a biomarker for congenital Chagas disease, using a screen-printed electrode modified with gold nanoparticles and functionalized with shed acute phase antigen

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• 作者：Matías Regiart ; Sirley V. Pereira ; Franco A. Bertolino…
• 关键词：Sandwich immunoassay ; Recombinant antigen ; Scanning electron microscopy ; Cyclic voltammetry ; X ; ray diffraction ; Horseradish peroxidase ; Serology
• 刊名：Microchimica Acta
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：183
• 期：3
• 页码：1203-1210
• 全文大小：758 KB
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• 作者单位：Matías Regiart (1)
  Sirley V. Pereira (1)
  Franco A. Bertolino (1)
  Carlos D. Garcia (2)
  Julio Raba (1)
  Pedro R. Aranda (1)
  
  1. INQUISAL, Department of Chemistry, National University of San Luis, CONICET, Chacabuco 917, D5700BW, San Luis, Argentina
  2. Department of Chemistry, Clemson University, Clemson, SC, SC 29631, USA
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Analytical Chemistry
  Inorganic Chemistry
  Physical Chemistry
  Characterization and Evaluation Materials
  Monitoring, Environmental Analysis and Environmental Ecotoxicology
  
• 出版者：Springer Wien
• ISSN：1436-5073

文摘

The authors describe a sandwich immunosensor for anti-IgM T. cruzi-specific antibodies which are a biomarker for congenital Chagas disease (CCD). A screen-printed carbon electrode (SPCE) was modified with gold nanoparticles (AuNPs) and then functionalized with recombinant shed acute phase antigen (SAPA). The AuNPs/SPCE was characterized by scanning electron microscopy, cyclic voltammetry, and X-ray diffraction. The AuNPs provide an increased active surface area, high conductivity, and improved electrocatalytic characteristics, this resulting in a significant improvement of the detection limit. Following interaction between immobilized SAPA and the target antibodies in the serum sample, secondary antibody (an anti-human IgM-HRP conjugate) and solutions of H₂O₂ and 4-tert-butylcatechol were added and the enzymatic product was detected at −100 mV (vs. Ag/SPCE). The resulting current was proportional to the anti-T. cruzi IgM antibodies present in the sample and showed a linear trend from 10 to 200 ng mL−1 (R = 0.998). The detection limit was 3.03 ng mL−1. These values demonstrate that this electrochemical immunosensor can be used as an alternative serological method for diagnosis of congenital Chagas disease.