Improved electrochemiluminescence labels for heterogeneous microbead immunoassay

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• 作者：Linpo Yu ; Yang Liu ; Ming Zhou
• 关键词：Electrochemiluminescence ; Immunoassay ; Ruthenium complexes ; Biolabels
• 刊名：Analytical and Bioanalytical Chemistry
• 出版年：2016
• 出版时间：October 2016
• 年：2016
• 卷：408
• 期：25
• 页码：7095-7103
• 全文大小：685 KB
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Analytical Chemistry
  Food Science
  Inorganic Chemistry
  Physical Chemistry
  Monitoring, Environmental Analysis and Environmental Ecotoxicology
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1618-2650
• 卷排序：408

文摘

Ruthenium(II) complexes with carboxylic acid as a bioconjugatable group, i.e., [Ru(bathophenanthroline disulfonate)(2,2′-bipyridine)(4-methyl-4′-(3-carboxypropyl)-2,2′-bipyridine)]0, (C₄₉H₃₈N₆O₈S₂Ru), and [Ru(bathophenanthroline disulfonate)₂(4-methyl-4′-(3-carboxypropyl)-2,2′-bipyridine)]2− · 2Na+, (C₆₃H₄₄N₆O₁₄S₄RuNa₂) were characterized spectroscopically and electrochemically. As potential labels for electrochemiluminescence (ECL) immunoassays, the ECL intensities of the free labels in homogenous aqueous buffer solutions were compared under a condition that is similar to the one employed by a commercial clinical immunoassay system. The two labels were found to be more emissive and, thus, can be detected at 10- 12 pM compared with 5× 10−12 pM of the label currently used in the commercial ECL system. Furthermore, the improved ECL emission of the free labels in homogenous solutions was proven to be translated into more intense ECL signal in heterogeneous sandwich immunoassay and, thus, leading to a lower limit of detection in immunoassay. The data obtained from these ECL labels shed light on the further development of ECL-based clinical immunoassay technology.