Evaluation of the contribution of the transmembrane region to the ectodomain conformation of the human immunodeficiency virus (HIV-1) envelope glycoprotein

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• 作者：Hanh T. Nguyen ; Navid Madani ; Haitao Ding ; Emerald Elder…
• 关键词：HIV ; 1 Env ; gp41 ; Transmembrane region ; Stabilize ; Ectodomain ; Fibritin –Trimer
• 刊名：Virology Journal
• 出版年：2017
• 出版时间：December 2017
• 年：2017
• 卷：14
• 期：1
• 全文大小：2432KB
• 刊物主题：Virology;
• 出版者：BioMed Central
• ISSN：1743-422X
• 卷排序：14

文摘

BackgroundThe human immunodeficiency virus (HIV-1) envelope glycoprotein (Env), a Type 1 transmembrane protein, assembles into a trimeric spike complex that mediates virus entry into host cells. The high potential energy of the metastable, unliganded Env trimer is maintained by multiple non-covalent contacts among the gp120 exterior and gp41 transmembrane Env subunits. Structural studies suggest that the gp41 transmembrane region forms a left-handed coiled coil that contributes to the Env trimer interprotomer contacts. Here we evaluate the contribution of the gp41 transmembrane region to the folding and stability of Env trimers.