Competition between Burkholderia pseudomallei and B. thailandensis

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• 作者：Wikanda Ngamdee (1)
  Sarunporn Tandhavanant (1) (2)
  Chanthiwa Wikraiphat (2)
  Onrapak Reamtong (3)
  Vanaporn Wuthiekanun (2)
  Jeanne Salje (2)
  David A Low (4) (5)
  Sharon J Peacock (1) (2) (6)
  Narisara Chantratita (1) (2)
  
  1. Department of Microbiology and Immunology ; Faculty of Tropical Medicine ; Mahidol University ; 420/6 Rajvithi Road ; Bangkok ; 10400 ; Thailand
  2. Mahidol-Oxford Tropical Medicine Research Unit ; Faculty of Tropical Medicine ; Mahidol University ; Bangkok ; Thailand
  3. Department of Molecular Tropical Medicine and Genetics ; Faculty of Tropical Medicine ; Mahidol University ; Bangkok ; Thailand
  4. Department of Molecular ; Cellular ; and Developmental Biology ; University of California ; Santa Barbara ; CA ; USA
  5. Biomolecular Science and Engineering Program ; University of California ; Santa Barbara ; CA ; USA
  6. Department of Medicine ; University of Cambridge ; Addenbrooke鈥檚 Hospital ; Cambridge ; UK
  
• 关键词：Burkholderia pseudomallei ; B. thailandensis ; Melioidosis ; Swarming ; Flagella ; Competitive growth inhibition
• 刊名：BMC Microbiology
• 出版年：2015
• 出版时间：December 2015
• 年：2015
• 卷：15
• 期：1
• 全文大小：3,462 KB
• 参考文献：1. Limmathurotsakul, D, Dance, DA, Wuthiekanun, V, Kaestli, M, Mayo, M, Warner, J (2013) Systematic review and consensus guidelines for environmental sampling of Burkholderia pseudomallei. PLoS Negl Trop Dis 7: pp. e2105 CrossRef
  2. Limmathurotsakul, D, Kanoksil, M, Wuthiekanun, V, Kitphati, R, DeStavola, B, Day, NP (2013) Activities of daily living associated with acquisition of melioidosis in northeast Thailand: a matched case鈥揷ontrol study. PLoS Negl Trop Dis 7: pp. e2072 CrossRef
  3. Wiersinga, WJ, Currie, BJ, Peacock, SJ (2012) Melioidosis. N Engl J Med 367: pp. 1035-44 CrossRef
  4. Chantratita, N, Wuthiekanun, V, Limmathurotsakul, D, Vesaratchavest, M, Thanwisai, A, Amornchai, P (2008) Genetic diversity and microevolution of Burkholderia pseudomallei in the environment. PLoS Negl Trop Dis 2: pp. e182 CrossRef
  5. Brett, PJ, Deshazer, D, Woods, DE (1997) Characterization of Burkholderia pseudomallei and Burkholderia pseudomallei-like strains. Epidemiol Infect 118: pp. 137-48 CrossRef
  6. Smith, MD, Angus, BJ, Wuthiekanun, V, White, NJ (1997) Arabinose assimilation defines a nonvirulent biotype of Burkholderia pseudomallei. Infect Immun 65: pp. 4319-21
  7. Trakulsomboon, S, Vuddhakul, V, Tharavichitkul, P, Na-Gnam, N, Suputtamongkol, Y, Thamlikitkul, V (1999) Epidemiology of arabinose assimilation in Burkholderia pseudomallei isolated from patients and soil in Thailand. Southeast Asian J Trop Med Public Health 30: pp. 756-9
  8. Duerkop, BA, Varga, J, Chandler, JR, Peterson, SB, Herman, JP, Churchill, ME (2009) Quorum-sensing control of antibiotic synthesis in Burkholderia thailandensis. J Bacteriol 191: pp. 3909-18 CrossRef
  9. Schwarz, S, West, TE, Boyer, F, Chiang, WC, Carl, MA, Hood, RD (2010) Burkholderia type VI secretion systems have distinct roles in eukaryotic and bacterial cell interactions. PLoS Pathog 6: pp. e1001068 CrossRef
  10. Nikolakakis, K, Amber, S, Wilbur, JS, Diner, EJ, Aoki, SK, Poole, SJ (2012) The toxin/immunity network of Burkholderia pseudomallei contact-dependent growth inhibition (CDI) systems. Mol Microbiol 84: pp. 516-29 CrossRef
  11. Anderson, MS, Garcia, EC, Cotter, PA (2012) The Burkholderia bcpAIOB genes define unique classes of two-partner secretion and contact dependent growth inhibition systems. PLoS Genet 8: pp. e1002877 CrossRef
  12. Chantratita, N, Wuthiekanun, V, Boonbumrung, K, Tiyawisutsri, R, Vesaratchavest, M, Limmathurotsakul, D (2007) Biological relevance of colony morphology and phenotypic switching by Burkholderia pseudomallei. J Bacteriol 189: pp. 807-17 CrossRef
  13. Tandhavanant, S, Thanwisai, A, Limmathurotsakul, D, Korbsrisate, S, Day, NP, Peacock, SJ (2010) Effect of colony morphology variation of Burkholderia pseudomallei on intracellular survival and resistance to antimicrobial environments in human macrophages in vitro. BMC Microbiol 10: pp. 303 CrossRef
  14. Manivanh, L, Pierret, A, Rattanavong, S, Buisson, Y, Elliott, I, Maeght, J-L (2013) Burkholderia pseudomallei in a rice paddy in Northern Laos-influence of depth, season and physicochemical parameters. 7th World Melioidosis Congress 2013, Bangkok, Thailand
  15. Kaestli, M, Schmid, M, Mayo, M, Rothballer, M, Harrington, G, Richardson, L (2012) Out of the ground: aerial and exotic habitats of the melioidosis bacterium Burkholderia pseudomallei in grasses in Australia. Environ Microbiol 14: pp. 2058-70 CrossRef
  16. Boonbumrung, K, Wuthiekanun, V, Rengpipat, S, Day, NP, Peacock, SJ (2006) In vitro motility of a population of clinical Burkholderia pseudomallei isolates. J Med Assoc Thai 89: pp. 1506-10
  17. DeShazer, D, Brett, PJ, Carlyon, R, Woods, DE (1997) Mutagenesis of Burkholderia pseudomallei with Tn5-OT182: isolation of motility mutants and molecular characterization of the flagellin structural gene. J Bacteriol 179: pp. 2116-25
  18. Vesaratchavest, M, Tumapa, S, Day, NP, Wuthiekanun, V, Chierakul, W, Holden, MT (2006) Nonrandom distribution of Burkholderia pseudomallei clones in relation to geographical location and virulence. J Clin Microbiol 44: pp. 2553-7 CrossRef
  19. Winstanley, C, Morgan, JA (1997) The bacterial flagellin gene as a biomarker for detection, population genetics and epidemiological analysis. Microbiology 143: pp. 3071-84 CrossRef
  20. Wajanarogana, S, Sonthayanon, P, Wuthiekanun, V, Panyim, S, Simpson, AJ, Tungpradabkul, S (1999) Stable marker on flagellin gene sequences related to arabinose non-assimilating pathogenic Burkholderia pseudomallei. Microbiol Immunol 43: pp. 995-1001 CrossRef
  21. Scott, AE, Twine, SM, Fulton, KM, Titball, RW, Essex-Lopresti, AE, Atkins, TP (2011) Flagellar glycosylation in Burkholderia pseudomallei and Burkholderia thailandensis. J Bacteriol 193: pp. 3577-87 CrossRef
  22. Holden, MT, Titball, RW, Peacock, SJ, Cerdeno-Tarraga, AM, Atkins, T, Crossman, LC (2004) Genomic plasticity of the causative agent of melioidosis, Burkholderia pseudomallei. Proc Natl Acad Sci U S A 101: pp. 14240-5 CrossRef
  23. Garcia, EC, Anderson, MS, Hagar, JA, Cotter, PA (2013) Burkholderia BcpA mediates biofilm formation independently of interbacterial contact-dependent growth inhibition. Mol Microbiol 89: pp. 1213-25 CrossRef
  24. Wuthiekanun, V, Limmathurotsakul, D, Chantratita, N, Feil, EJ, Day, NP, Peacock, SJ (2009) Burkholderia pseudomallei is genetically diverse in agricultural land in Northeast Thailand. PLoS Negl Trop Dis 3: pp. e496 CrossRef
  25. Laemmli, UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: pp. 680-5 CrossRef
  26. De Jong IG, Beilharz K, Kuipers OP, Veening JW. Live cell imaging of / Bacillus subtilis and / Streptococcus pneumoniae using automated time-lapse microscopy. J Vis Exp. 2011(53). doi: 10.3791/3145.
  27. Chantratita, N, Tandhavanant, S, Wikraiphat, C, Trunck, LA, Rholl, DA, Thanwisai, A (2012) Proteomic analysis of colony morphology variants of Burkholderia pseudomallei defines a role for the arginine deiminase system in bacterial survival. J Proteomics 75: pp. 1031-42 CrossRef
  
• 刊物主题：Microbiology; Biological Microscopy; Fungus Genetics; Parasitology; Virology; Life Sciences, general;
• 出版者：BioMed Central
• ISSN：1471-2180

文摘

Background Burkholderia pseudomallei is a Gram-negative bacterium that causes melioidosis, an often fatal disease in tropical countries. Burkholderia thailandensis is a non-virulent but closely related species. Both species are soil saprophytes but are almost never isolated together. Results We identified two mechanisms by which B. pseudomallei affects the growth of B. thailandensis. First, we found that six different isolates of B. pseudomallei inhibited the growth of B. thailandensis on LB agar plates. Second, our results indicated that 55% of isolated strains of B. pseudomallei produced a secreted compound that inhibited the motility but not the viability of B. thailandensis. Analysis showed that the active compound was a pH-sensitive and heat-labile compound, likely a protein, which may affect flagella processing or facilitate their degradation. Analysis of bacterial sequence types (STs) demonstrated an association between this and motility inhibition. The active compound was produced from B. pseudomallei during the stationary growth phase. Conclusion Taken together, our results indicate that B. pseudomallei inhibits both the growth and motility of its close relative B. thailandensis. The latter phenomenon appears to occur via a previously unreported mechanism involving flagellar processing or degradation.