Nanoparticles-based nanochannels assembled on a plastic flexible substrate for label-free immunosensing

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• 作者：Alfredo de la Escosura-Mu?iz ; Marisol Espinoza-Casta?eda ; Madoka Hasegawa…
• 关键词：nanochannel ; dip ; coating ; electrochemical biosensor ; label ; free immunosensing
• 刊名：Nano Research
• 出版年：2015
• 出版时间：April 2015
• 年：2015
• 卷：8
• 期：4
• 页码：1180-1188
• 全文大小：1,746 KB
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• 作者单位：Alfredo de la Escosura-Mu?iz (1)
  Marisol Espinoza-Casta?eda (1)
  Madoka Hasegawa (2)
  Laetitia Philippe (2)
  Arben Merko?i (1) (3)
  
  1. ICN2-Nanobioelectronics & Biosensors Group, Institut Catala de Nanociencia i Nanotecnologia, Campus UAB, 08193, Bellaterra (Barcelona), Spain
  2. EMPA, Swiss Federal Laboratories for Materials Science and Technology, Feuerwerkerstrasse 39, 3602, Thun, Switzerland
  3. ICREA-Institucio Catalana de Recerca i Estudis Avan?ats, 08010, Barcelona, Spain
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chinese Library of Science
  Chemistry
  Nanotechnology
  
• 出版者：Tsinghua University Press, co-published with Springer-Verlag GmbH
• ISSN：1998-0000

文摘

A novel, cheap, disposable and single-use nanoparticles-based nanochannel platform assembled on a flexible substrate for label-free immunosensing is presented. This sensing platform is formed by the dip-coating of a homogeneous and assembled monolayer of carboxylated polystyrene nanospheres (PS, 200 and 500 nm-sized) onto the working area of flexible screen-printed indium tin oxide/polyethylene terephthalate (ITO/PET) electrodes. The spaces between the self-assembled nanospheres generate well-ordered nanochannels, with inter-PS particles distances of around 65 and 24 nm respectively. The formed nanochannels are used for the effective immobilization of antibodies and subsequent protein detection based on the monitoring of [Fe(CN)₆]4?/sup> flow through diffusion and the decrease in the differential pulse voltammetric signal upon immunocomplex formation. The obtained sensing system is nanochannel-size dependent and allows human immunoglobulin G (IgG) (chosen as a model analyte) to be detected at levels of 580 ng/mL. The system also exhibits an excellent specificity against other proteins present in real samples and shows good performance with a human urine sample. The developed device represents an integrated and simple biodetection system which overcomes many of the limitations of previously reported nanochannels-based approaches and can be extended in the future to several other immuno and DNA detection systems.