Enhancement of biosensing performance using a polyaniline/multiwalled carbon nanotubes nanocomposite

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• 作者：Hoang Thi Hien ; Ho Truong Giang ; Tran Trung ; Chu Van Tuan
• 刊名：Journal of Materials Science
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：52
• 期：3
• 页码：1694-1703
• 全文大小：
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Materials Science, general; Characterization and Evaluation of Materials; Polymer Sciences; Continuum Mechanics and Mechanics of Materials; Crystallography and Scattering Methods; Classical Mechanics;
• 出版者：Springer US
• ISSN：1573-4803
• 卷排序：52

文摘

In this study, an effective electrochemical method was successfully developed to produce polyaniline/multiwalled carbon nanotubes nanocomposite on interdigitated platinum microelectrodes for the enhancement of biosensing performance. Morphology and structure of nanocomposite were investigated by field emission scanning electron microscopy and ultraviolet–visible spectroscopy. Fourier transform infrared spectroscopy technique was used to identify the presence of polyaniline/multiwalled carbon nanotubes on the surface of microelectrodes. IgG polyclonal antibodies against Japanese encephalitis virus (JEV) were immobilized onto nanocomposite-modified microelectrodes, acting as an electrochemical immunosensor for label-free detection of JEV antigens. Results showed that the linear detection range of the immunosensor for JEV antigens was 2–250 ng/mL. The electrochemical impedance spectroscopy analysis also indicated that a negligible response was found when the immunosensor exposed to non-specific molecules. This work showed the potential use of polyaniline/multiwalled carbon nanotubes nanocomposite in the platform of electrochemical immunosensors for label-free detection of pathogens and small biomolecules.