Performance and lifetime of slurry packed capillary columns for high performance liquid chromatography

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• 作者：Martin Franc ; Ji?í Vojta ; Jana Sobotníková ; Pavel Coufal…
• 关键词：high performance liquid chromatography ; slurry packing ; Van Deemter curve ; separation impedance
• 刊名：Chemical Papers
• 出版年：2014
• 出版时间：January 2014
• 年：2014
• 卷：68
• 期：1
• 页码：22-28
• 全文大小：555KB
• 参考文献：1. Andreolini, F., Borra, C., & Novotny, M. (1987). Preparation and evaluation of slurry packed capillary columns for normal-phase liquid chromatography. / Analytical Chemistry, / 59, 2428-432. DOI: 10.1021/ac00146a023. CrossRef
  2. Angus, P. D. A., Demarest, C.W., Catalano, T., & Stobaugh, J. F. (2000). Aspects of column fabrication for packed capillary electrochromatography. / Journal of Chromatography A, / 887, 347-65. DOI: 10.1016/s0021-9673(00)00529-x. CrossRef
  3. Borra, C., Han, S. M., & Novotny, M. (1987). Quantitative analytical aspects of reversed-phase liquid chromatography with slurry-packed capillary columns. / Journal of Chromatography A, / 385, 75-5. DOI: 10.1016/s0021-9673(01)94623-0. CrossRef
  4. Bristow, P. A., & Knox, J. H. (1977). Standardization of test conditions for high performance liquid chromatography columns. / Chromatographia, / 10, 279-89. DOI: 10.1007/ bf02263001. CrossRef
  5. Bristow, P. A., Brittain, P. N., Riley, C. M., & Williamson, B. F. (1977). Upward slurry packing of liquid chromatography columns. / Journal of Chromatography A, / 131, 57-4. DOI: 10.1016/s0021-9673(00)80920-6. CrossRef
  6. Cortes, H. J., & Pfeiffer, C. D. (1993). Microcolumn size exclusion chromatography with polymeric stationary phases. / Analytical Chemistry, / 65, 1476-480. DOI: 10.1021/ac00058a028. CrossRef
  7. Crescentini, G., Bruner, F., Mangani, F., & Yafeng, G. (1988). Preparation and evaluation of dry-packed capillary columns for high-performance liquid chromatography. / Analytical Chemistry, / 60, 1659-662. DOI: 10.1021/ac00168a005. CrossRef
  8. Ehlert, S., R?sler, T., & Tallarek, U. (2008a). Packing density of slurry-packed capillaries at low aspect ratios. / Journal of Separation Science, / 31, 1719-728. DOI: 10.1002/jssc.200800018. CrossRef
  9. Ehlert, S., Kraiczek, K., Mora, J. A., Dittmann, M., Rozing, G. P., & Tallarek, U. (2008b). Separation efficiency of particlepacked HPLC microchips. / Analytical Chemistry, / 80, 5945-950. DOI: 10.1021/ac800576v. CrossRef
  10. Gaspar, A., Piyasena, M. E., & Gomez, F. A. (2007). Fabrication of fritless chromatographic microchips packed with conventional reversed-phase silica particles. / Analytical Chemistry, / 79, 7906-909. DOI: 10.1021/ac071106g. CrossRef
  11. Gluckman, J. C., Hirose, A., McGuffin, V. L., & Novotny, M. (1983). Performance evaluation of slurry-packed capillary columns for liquid chromatography. / Chromatographia, / 17, 303-09. DOI: 10.1007/bf02270662. CrossRef
  12. Jung, S., Ehlert, S., Mora, J. A., Kraiczek, K., Dittmann, M., Rozing, G. P., & Tallarek, U. (2009). Packing density, permeability, and separation efficiency of packed microchips at different particle-aspect ratios. / Journal of Chromatography A, / 1216, 264-73. DOI: 10.1016/j.chroma.2008.11.073. CrossRef
  13. Karlsson, K. E., & Novotny, M. (1988). Separation efficiency of slurry-packed liquid chromatography microcolumns with very small inner diameters. / Analytical Chemistry, / 60, 1662-665. DOI: 10.1021/ac00168a006. CrossRef
  14. Keller, H. P., Erni, F., Linder, H. R., & Frei, R. W. (1977). Dynamic slurry-packing technique for liquid chromatography columns. / Analytical Chemistry, / 49, 1958-963. DOI: 10.1021/ac50021a018. CrossRef
  15. Kennedy, R. T., & Jorgenson, J. W. (1989). Preparation and evaluation of packed capillary liquid chromatography columns with inner diameters from 20 to 50 micrometers. / Analytical Chemistry, / 61, 1128-135. DOI: 10.1021/ac00185a016. CrossRef
  16. Kirkland, J. J., & DeStefano, J. J. (2006). The art and science of forming packed analytical high-performance liquid chromatography columns. / Journal of Chromatography A, / 1126, 50-7. DOI: 10.1016/j.chroma.2006.04.027. CrossRef
  17. Konishi, M., Mori, Y., & Amano, T. (1985). High-performance packed glass-lined stainless steel capillary column for microcolumn liquid chromatography. / Analytical Chemistry, / 57, 2235-239. DOI: 10.1021/ac00289a014. CrossRef
  18. Lazar, I. M., Trisiripisal, P., & Sarvaiya, H. A. (2006). Microfluidic liquid chromatography system for proteomic applications and biomarker screening. / Analytical Chemistry, / 78, 5513-524. DOI: 10.1021/ac060434y. CrossRef
  19. Leonardis, I., Capriotti, F., Cappiello, A., Famiglini, G., & Palma, P. (2012). Temperature effects on nano-LC column packing technology. / Journal of Separation Science, / 35, 1589-595. DOI: 10.1002/jssc.201200081. CrossRef
  20. Lottes, F., Arlt, W., Minceva, M., & Stenby, E. H. (2009). Hydrodynamic impact of particle shape in slurry packed liquid chromatography columns. / Journal of Chromatography A, / 1216, 5687-695. DOI: 10.1016/j.chroma.2009.05.090. CrossRef
  21. Lynen, F., Buica, A., de Villiers, A., Crouch, A., & Sandra, P. (2005). An efficient slurry packing procedure for the preparation of columns applicable in capillary electrochromatography and capillary electrochromatography-electrospray-mass spectrometry. / Journal of Separation Science, / 28, 1539-549. DOI: 10.1002/jssc.200400099. CrossRef
  22. Maloney, T. D., & Colón, L. A. (2002). Comparison of column packing techniques for capillary electrochromatography. / Journal of Separation Science, / 25, 1215-225. DOI: 10.1002/1615-9314(20021101)25:15/17?215::aid-jssc1215?.0.co;2-o. CrossRef
  23. Meyer, R. F., & Hartwick, R. A. (1984). Efficient packing of small particle microbore columns. / Analytical Chemistry, / 56, 2211-214. DOI: 10.1021/ac00276a051. CrossRef
  24. Robson, M. M., Roulin, S., Shariff, S. M, Raynor, M. W., Bartle, K. D., Clifford, A. A., Myers, P., Euerby, M. R., & Johnson, C. M. (1996). Capillary electrochromatography using columns packed with a supercritical fluid carrier. / Chromatographia, / 43, 313-21. DOI: 10.1007/bf02271002. CrossRef
  25. Roulin, S., Dmoch, R., Carney, R., Bartle, K. D., Myers, P., Euerby, M. R., & Johnson, C. (2000). Comparison of different packing methods for capillary electrochromatography columns. / Journal of Chromatography A, / 887, 307-12. DOI: 10.1016/s0021-9673(00)00366-6. CrossRef
  26. Shen, Y., Yang, Y. J., & Lee, M. L. (1997). Fundamental considerations of packed-capillary GC, SFC, and LC using nonporous silica particles. / Analytical Chemistry, / 69, 628-35. DOI: 10.1021/ac960657w. CrossRef
  27. Vissers, J. P. C., Claessens, H. A., Laven, J., & Cramers, C. A. (1995). Colloid chemical aspects of slurry packing techniques in microcolumn liquid chromatography. / Analytical Chemistry, / 67, 2103-109. DOI: 10.1021/ac00109a032. CrossRef
  28. Wong, V., Shalliker, R. A., & Guiochon, G. (2004). Evaluation of the uniformity of analytical-size chromatography columns prepared by the downward packing of particulate slurries. / Analytical Chemistry, / 76, 2601-608. DOI: 10.1021/ac030391a. CrossRef
  29. Xie, J., Miao, Y., Shih, J., Tai, Y. C., & Lee, T. D. (2005). Microfluidic platform for liquid chromatography-tandem mass spectrometry analyses of complex peptide mixtures. / Analytical Chemistry, / 77, 6947-953. DOI: 10.1021/ac0510888. CrossRef
  30. Yin, H. F., Killeen, K., Brennen, R., Sobek, D., Werlich, M., & van de Goor, T. (2005). Microfluidic chip for peptide analysis with an integrated HPLC column, sample enrichment column, and nanoelectrospray tip. / Analytical Chemistry, / 77, 527-33. DOI: 10.1021/ac049068d. CrossRef
  31. Zimina, T., Smith, R. M., Highfield, J. C., Myers, P., & King, B. W. (1996). Study of the flow development during the slurry packing of microcolumns for liquid chromatography. / Journal of Chromatography A, / 728, 33-5. DOI: 10.1016/0021-9673(95)01026-2. CrossRef
  
• 作者单位：Martin Franc (1)
  Ji?í Vojta (1)
  Jana Sobotníková (1)
  Pavel Coufal (1)
  Zuzana Bosáková (1)
  
  1. Faculty of Science, Department of Analytical Chemistry, Charles University in Prague, Albertov 2030, 128 43, Prague, Czech Republic
  
• ISSN：1336-9075

文摘

Fused silica capillary columns of the internal diameter of 320 μm were packed with the Nucleosil C18 stationary phase of 5 μm using the slurry packing method. The time of the bed compaction phase, packing pressure, and the use of ultrasound varied to study their influence on the column performance. Van Deemter curves were measured and separation impedance values were calculated in order to assess both separation efficiency and kinetic performance of the columns. Selected columns were tested again after nine months to evaluate the stability of their beds. Separation efficiencies of all columns were similar, but a major difference, caused by the use of ultrasound, was observed in the bed stability. Columns sonicated for 25 minutes during the bed compaction phase exhibited unchanged performance in the course of several months, while the performance of non-sonicated columns decreased.