A Scutellaria baicalensis R2R3-MYB gene, SbMYB8, regulates flavonoid biosynthesis and improves drought stress tolerance in transgenic tobacco

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• 作者：Yuan Yuan (1)
  Linjie Qi (1)
  Jian Yang (1)
  Chong Wu (1)
  Yunjun Liu (2)
  Luqi Huang (1)
  
  1. State Key Laboratory of Dao-di Herbs ; National Resource Center for Chinese Materia Medica ; Academy of Chinese Medical Sciences ; Beijing ; 100700 ; China
  2. Institute of Crop Science ; Chinese Academy of Agricultural Sciences ; Beijing ; 100081 ; China
  
• 关键词：R2R3 ; MYB protein ; SbMYB8 ; Medicinal plant ; Flavonoid biosynthesis ; Stress tolerance ; Transgenic tobacco
• 刊名：Plant Cell, Tissue and Organ Culture
• 出版年：2015
• 出版时间：March 2015
• 年：2015
• 卷：120
• 期：3
• 页码：961-972
• 全文大小：1,548 KB
• 参考文献：1. Abe, H, Urao, T, Ito, T, Seki, M, Shinozaki, K, Yamaguchi-Shinozaki, K (2003) Arabidopsis AtMYC2 (bHLH) and AtMYB2 (MYB) function as transcriptional activators in abscisic acid signaling. Plant Cell 15: pp. 63-78 CrossRef
  2. Bartels, D, Sunkar, R (2005) Drought and salt tolerance in plants. Crit Rev Plant Sci 24: pp. 23-58 CrossRef
  3. Beyer, W, Fridovich, I (1987) Assaying for superoxide dismutase activity: some large consequences of minor changes in conditions. Anal Biochem 161: pp. 559-566 CrossRef
  4. Blach-Olszewska, Z, Jatczak, B, Rak, A, Lorenc, M, Gulanowski, B, Drobna, A, Lamer-Zarawska, E (2008) Production of cytokines and stimulation of resistance to viral infection in human leukocytes by Scutellaria baicalensis flavones. J Interferon Cytokine Res 28: pp. 571-581 CrossRef
  5. Booij-James, IS, Dube, SK, Jansen, MA, Edelman, M, Mattoo, AK (2000) Ultraviolet-B radiation impacts light-mediated turnover of the photosystem II reaction center heterodimer in Arabidopsis mutants altered in phenolic metabolism. Plant Physiol 124: pp. 1275-1284 CrossRef
  6. Bradford, MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: pp. 248-254 CrossRef
  7. Butelli, E, Titta, L, Giorgio, M, Mock, HP, Matros, A, Peterek, S, Schijlen, EG, Hall, RD, Bovy, AG, Luo, J (2008) Enrichment of tomato fruit with health-promoting anthocyanins by expression of select transcription factors. Nat Biotechnol 26: pp. 1301-1308 CrossRef
  8. Chance, B, Maehly, A (1955) Assay of catalases and peroxidases. Methods Enzymol 2: pp. 764-775 CrossRef
  9. Cheng, YJ, Kim, MD, Deng, XP, Kwak, SS, Chen, W (2013) Enhanced salt stress tolerance in transgenic potato plants expressing IbMYB1, a sweet potato transcription factor. J Microbiol Biotechnol 23: pp. 1737-1746 CrossRef
  10. Cui, MH, Yoo, KS, Hyoung, S, Nguyen, HT, Kim, YY, Kim, HJ, Ok, SH, Yoo, SD, Shin, JS (2013) An Arabidopsis R2R3-MYB transcription factor, AtMYB20, negatively regulates type 2C serine/threonine protein phosphatases to enhance salt tolerance. FEBS Lett 587: pp. 1773-1778 CrossRef
  11. Ding, Z, Li, S, An, X, Liu, X, Qin, H, Wang, D (2009) Transgenic expression of MYB15 confers enhanced sensitivity to abscisic acid and improved drought tolerance in Arabidopsis thaliana. J Genet Genomics 36: pp. 17-29 CrossRef
  12. Espley, RV, Hellens, RP, Putterill, J, Stevenson, DE, Kutty-Amma, S, Allan, AC (2007) Red colouration in apple fruit is due to the activity of the MYB transcription factor, MdMYB10. Plant J 49: pp. 414-427 CrossRef
  13. Fini, A, Brunetti, C, Ferdinando, M, Ferrini, F, Tattini, M (2011) Stress-induced flavonoid biosynthesis and the antioxidant machinery of plants. Plant Signal Behav 6: pp. 709-711 CrossRef
  14. Gonzalez, A, Zhao, M, Leavitt, JM, Lloyd, AM (2008) Regulation of the anthocyanin biosynthetic pathway by the TTG1/bHLH/Myb transcriptional complex in Arabidopsis seedlings. Plant J 53: pp. 814-827 CrossRef
  15. Harborne, JB, Williams, CA (2000) Advances in flavonoid research since 1992. Phytochemistry 55: pp. 481-504 CrossRef
  16. He, Y, Li, W, Lv, J, Jia, Y, Wang, M, Xia, G (2012) Ectopic expression of a wheat MYB transcription factor gene, TaMYB73, improves salinity stress tolerance in Arabidopsis thaliana. J Exp Bot 63: pp. 1511-1522 CrossRef
  17. Horsch, RB, Rogers, SG, Fraley, RT (1985) Transgenic plants. Cold Spring Harb Symp Quant Biol 50: pp. 433-437 CrossRef
  18. Huang, W, Sun, W, Lv, H, Luo, M, Zeng, S, Pattanaik, S, Yuan, L, Wang, Y (2013) A R2R3-MYB transcription factor from Epimedium sagittatum regulates the flavonoid biosynthetic pathway. PLoS One 8: pp. e70778 CrossRef
  19. Kaur, H, Heinzel, N, Schottner, M, Baldwin, IT, Galis, I (2010) R2R3-NaMYB8 regulates the accumulation of phenylpropanoid-polyamine conjugates, which are essential for local and systemic defense against insect herbivores in Nicotiana attenuata. Plant Physiol 152: pp. 1731-1747 CrossRef
  20. Kranz, HD, Denekamp, M, Greco, R, Jin, H, Leyva, A, Meissner, RC, Petroni, K, Urzainqui, A, Bevan, M, Martin, C (1998) Towards functional characterisation of the members of the R2R3-MYB gene family from Arabidopsis thaliana. Plant J 16: pp. 263-276 CrossRef
  21. Lee, TG, Jang, CS, Kim, JY, Kim, DS, Park, JH, Kim, DY, Seo, YW (2007) A Myb transcription factor (TaMyb1) from wheat roots is expressed during hypoxia: roles in response to the oxygen concentration in root environment and abiotic stresses. Physiol Plant 129: pp. 375-385 CrossRef
  22. Li, D, Zhang, Y, Hu, X, Shen, X, Ma, L, Su, Z, Wang, T, Dong, J (2011) Transcriptional profiling of Medicago truncatula under salt stress identified a novel CBF transcription factor MtCBF4 that plays an important role in abiotic stress responses. BMC Plant Biol 11: pp. 109 CrossRef
  23. Luo, J, Butelli, E, Hill, L, Parr, A, Niggeweg, R, Bailey, P, Weisshaar, B, Martin, C (2008) AtMYB12 regulates caffeoyl quinic acid and flavonol synthesis in tomato: expression in fruit results in very high levels of both types of polyphenol. Plant J 56: pp. 316-326 CrossRef
  24. Mahajan, S, Tuteja, N (2005) Cold, salinity and drought stresses: an overview. Arch Biochem Biophys 444: pp. 139-158 CrossRef
  25. Mao, X, Jia, D, Li, A, Zhang, H, Tian, S, Zhang, X, Jia, J, Jing, R (2011) Transgenic expression of TaMYB2A confers enhanced tolerance to multiple abiotic stresses in Arabidopsis. Funct Integr Genomics 11: pp. 445-465 CrossRef
  26. Meissner, RC, Jin, H, Cominelli, E, Denekamp, M, Fuertes, A, Greco, R, Kranz, HD, Penfield, S, Petroni, K, Urzainqui, A (1999) Function search in a large transcription factor gene family in Arabidopsis: assessing the potential of reverse genetics to identify insertional mutations in R2R3 MYB genes. Plant Cell 11: pp. 1827-1840 CrossRef
  27. Miller, G, Suzuki, N, Ciftci-Yilmaz, S, Mittler, R (2010) Reactive oxygen species homeostasis and signalling during drought and salinity stresses. Plant Cell Environ 33: pp. 453-467 CrossRef
  28. Morimoto, S, Tateishi, N, Matsuda, T, Tanaka, H, Taura, F, Furuya, N, Matsuyama, N, Shoyama, Y (1998) Novel hydrogen peroxide metabolism in suspension cells of Scutellaria baicalensis Georgi. J Biol Chem 273: pp. 12606-12611 CrossRef
  29. Nagashima, S, Hirotani, M, Yoshikawa, T (2000) Purification and characterization of UDP-glucuronate: baicalein 7-O-glucuronosyltransferase from Scutellaria baicalensis Georgi. cell suspension cultures. Phytochemistry 53: pp. 533-538 CrossRef
  30. Nakabayashi, R, Yonekura-Sakakibara, K, Urano, K, Suzuki, M, Yamada, Y, Nishizawa, T, Matsuda, F, Kojima, M, Sakakibara, H, Shinozaki, K (2014) Enhancement of oxidative and drought tolerance in Arabidopsis by overaccumulation of antioxidant flavonoids. Plant J 77: pp. 367-379 CrossRef
  31. Niu, SS, Xu, CJ, Zhang, WS, Zhang, B, Li, X, Lin-Wang, K, Ferguson, IB, Allan, AC, Chen, KS (2010) Coordinated regulation of anthocyanin biosynthesis in Chinese bayberry (Myrica rubra) fruit by a R2R3 MYB transcription factor. Planta 231: pp. 887-899 CrossRef
  32. Preston, J, Wheeler, J, Heazlewood, J, Li, SF, Parish, RW (2004) AtMYB32 is required for normal pollen development in Arabidopsis thaliana. Plant J 40: pp. 979-995 CrossRef
  33. Qin, Y, Wang, M, Tian, Y, He, W, Han, L, Xia, G (2012) Over-expression of TaMYB33 encoding a novel wheat MYB transcription factor increases salt and drought tolerance in Arabidopsis. Mol Biol Rep 39: pp. 7183-7192 CrossRef
  34. Rommens, CM, Richael, CM, Yan, H, Navarre, DA, Ye, J, Krucker, M, Swords, K (2008) Engineered native pathways for high kaempferol and caffeoylquinate production in potato. Plant Biotechnol J 6: pp. 870-886 CrossRef
  35. Sasaki, K, Taura, F, Shoyama, Y, Morimoto, S (2000) Molecular characterization of a novel beta-glucuronidase from Scutellaria baicalensis georgi. J Biol Chem 275: pp. 27466-27472
  36. Shan, H, Chen, S, Jiang, J, Chen, F, Chen, Y, Gu, C, Li, P, Song, A, Zhu, X, Gao, H (2012) Heterologous expression of the chrysanthemum R2R3-MYB transcription factor CmMYB2 enhances drought and salinity tolerance, increases hypersensitivity to ABA and delays flowering in Arabidopsis thaliana. Mol Biotechnol 51: pp. 160-173 CrossRef
  37. Sinha, AK (1972) Colorimetric assay of catalase. Anal Biochem 47: pp. 389-394 CrossRef
  38. Soltesz, A, Vagujfalvi, A, Rizza, F, Kerepesi, I, Galiba, G, Cattivelli, L, Coraggio, I, Crosatti, C (2012) The rice Osmyb4 gene enhances tolerance to frost and improves germination under unfavourable conditions in transgenic barley plants. J Appl Genet 53: pp. 133-143 CrossRef
  39. Winkel-Shirley, B (2002) Biosynthesis of flavonoids and effects of stress. Curr Opin Plant Biol 5: pp. 218-223 CrossRef
  40. Xu, H, Park, NI, Li, X, Kim, YK, Lee, SY, Park, SU (2010) Molecular cloning and characterization of phenylalanine ammonia-lyase, cinnamate 4-hydroxylase and genes involved in flavone biosynthesis in Scutellaria baicalensis. Bioresour Technol 101: pp. 9715-9722 CrossRef
  41. Yamaguchi-Shinozaki, K, Shinozaki, K (2006) Transcriptional regulatory networks in cellular responses and tolerance to dehydration and cold stresses. Annu Rev Plant Biol 57: pp. 781-803 CrossRef
  42. Yang, A, Dai, X, Zhang, WH (2012) A R2R3-type MYB gene, OsMYB2, is involved in salt, cold, and dehydration tolerance in rice. J Exp Bot 63: pp. 2541-2556 CrossRef
  43. Yuan Y, Hao J, Yang B, Li H, Li Z (2010) Climate change affected the best producing area of Chinese herbal medicine / Scutellaria baicalensis Georgi. J Tradit Med (Russia) 3聽s: 241鈥?48
  44. Yuan, Y, Liu, Y, Wu, C, Chen, S, Wang, Z, Yang, Z, Qin, S, Huang, L (2012) Water deficit affected flavonoid accumulation by regulating hormone metabolism in Scutellaria baicalensis Georgi roots. PLoS One 7: pp. e42946 CrossRef
  45. Yuan, Y, Liu, Y, Luo, Y, Huang, L, Chen, S, Yang, Z, Qin, S (2013) High temperature effects on flavones accumulation and antioxidant system in Scutellaria baicalensis Georgi cells. Afr J Biotechnol 10: pp. 5182-5192
  46. Yuan, Y, Wu, C, Liu, Y, Yang, J, Huang, L (2013) The Scutellaria baicalensis R2R3-MYB transcription factors modulates flavonoid biosynthesis by regulating GA metabolism in transgenic tobacco plants. PLoS One 8: pp. e77275 CrossRef
  47. Zhang, L, Zhao, G, Xia, C, Jia, J, Liu, X, Kong, X (2012) A wheat R2R3-MYB gene, TaMYB30-B, improves drought stress tolerance in transgenic Arabidopsis. J Exp Bot 63: pp. 5873-5885 CrossRef
  48. Zhang, Z, Liu, X, Wang, X, Zhou, M, Zhou, X, Ye, X, Wei, X (2012) An R2R3 MYB transcription factor in wheat, TaPIMP1, mediates host resistance to Bipolaris sorokiniana and drought stresses through regulation of defense- and stress-related genes. New Phytol 196: pp. 1155-1170 CrossRef
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Plant Sciences
  Plant Physiology
  
• 出版者：Springer Netherlands
• ISSN：1573-5044

文摘

R2R3-MYB proteins are involved in the primary and secondary metabolism, developmental processes and the responses to biotic and abiotic stresses. Little is known about the functions of R2R3-MYB proteins in Scutellaria baicalensis Georgi which is a traditional Chinese medicinal plants. In this study, the function of a S. baicalensis R2R3-MYB protein, SbMYB8, was investigated. SbMYB8 had similar expression pattern with SbC4H and SbCHS in ABA-treated S. baicalensis, indicating that SbMYB8 might be involved in the flavonoid metabolism. SbMYB8 protein could bind to the GmMYB92 BS3 sequence of SbCHS promoter region, regulating the expression of SbCHS. The SbMYB8 protein was localized to the nucleus where it activated transcription. The transgenic tobacco plants over-expressing SbMYB8 had higher caffeoylquinic acid contents, compared to that in wild type plants. Overexpression of SbMYB8 also changed the expression level of some flavonoid biosynthesis-related genes. It was found that overexpression of SbMYB8 can improve stress tolerance of transgenic plants, and can alter the activity and expression levels of some antioxidant enzymes. These results indicate that SbMYB8 plays important roles in flavonoid biosynthesis and stress tolerance of plant.