Preparative method for obtaining recombinant human interferon α2b from inclusion bodies of Escherichia coli
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- 作者：V. P. Romanov ; T. I. Kostromina…
- 关键词：recombinant α2b interferon ; inclusion bodies ; refolding ; protein isolation ; ion ; exchange chromatography ; reversed ; phase HPLC
- 刊名：Russian Journal of Bioorganic Chemistry
- 出版年：2016
- 出版时间：November 2016
- 年：2016
- 卷：42
- 期：6
- 页码：631-637
- 全文大小：
- 刊物类别：Biomedical and Life Sciences
- 刊物主题：Biochemistry, general; Bioorganic Chemistry; Organic Chemistry; Biomedicine general;
- 出版者：Pleiades Publishing
- ISSN：1608-330X
- 卷排序：42
文摘
A simple, easily reproducible, and scalable method for obtaining recombinant human interferon α2b from Escherichia coli inclusion bodies has been elaborated. It involves the following steps: preparation of producer cell biomass, isolation and washing of inclusion bodies, their dissolution with protein refolding, SP Sepharose chromatography, and DEAE Sepharose chromatography. According to the results of gel electrophoresis and reversed-phase HPLC, the purity of the protein obtained exceeds 95%.