Novel SNP identification in exon 3 of HSP90AA1 gene and their association with heat tolerance traits in Karan Fries (Bos taurus × Bos indicus) cows under tropical climatic condition

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• 作者：Rakesh Kumar ; Ishwar Dayal Gupta ; Archana Verma…
• 关键词：Karan Fries ; Heat stress ; HSP90AA1 ; SNP
• 刊名：Tropical Animal Health and Production
• 出版年：2016
• 出版时间：April 2016
• 年：2016
• 卷：48
• 期：4
• 页码：735-740
• 全文大小：338 KB
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• 作者单位：Rakesh Kumar (1)
  Ishwar Dayal Gupta (2)
  Archana Verma (2)
  Sohan Vir Singh (2)
  Nishant Verma (1)
  M. R. Vineeth (1)
  Ankit Magotra (3)
  Ramendra Das (1)
  
  1. Dairy Cattle Breeding Division, Indian Council of Agricultural Research, National Dairy Research Institute, Karnal, Haryana, 132001, India
  2. Principal Scientist, Dairy Cattle Breeding Division, Indian Council of Agricultural Research, National Dairy Research Institute, Karnal, Haryana, 132001, India
  3. Animal Genetics and Breeding Department, LUVAS, Hisar, Haryana, 125004, India
  
• 刊物类别：Medicine
• 刊物主题：Medicine & Public Health
  Veterinary medicine
  Zoology
  
• 出版者：Springer Netherlands
• ISSN：1573-7438

文摘

Heat shock proteins (HSPs) act as molecular chaperones those are preferentially transcribed in respose to heat stress and the polymorphism in HSP genes associated with heat tolerance traits in cows. HSP90AA1 gene has been mapped on Bos taurus autosome 21 (BTA-21) and spans nearly 5368 bp comprising of 11 exons out of which the first exon does not translate. The present study was done on Karan Fries (5/8 HF × 3/8 Tharparkar) cows reared in tropical climate with the objectives of identifying single-nucleotide polymorphisms (SNPs) in targeted regions (exon 3) of HSP90AA1 gene and analyzing their association with heat tolerance traits in Karan Fries cows. Respiration rate (RR) and rectal temperature (RT) were recorded once daily for four consecutive days during probable extreme hours in different seasons or temperature humidity index (THI), viz., winter, spring, and summer. For detecting single-nucleotide polymorphisms, sequence data were analyzed using BioEdit software (version 7.2). Comparative sequence analysis of HSP90AA1 gene showed point mutation, viz., g.1209A>G (exon 3) as compared to Bos taurus (NCBI Ref Seq: AC_000178.1). Association analysis indicated that THI was influenced (P < 0.01) by RR, RT, and HTC. Similarly, SNPs at locus g.1209A>G were categorized into three genotypes, i.e., AA, AG, and GG, and the least squares means (LSMEANS) of RR, RT, and HTC for GG (homozygous) genotype were significantly lower (P < 0.01) than AA (homozygous) and AG (heterozygous) genotypes. These findings may partly suggest that cows with GG genotypes were favored for heat tolerance trait, which can be used as an aid to selection for thermo-tolerance Karan Fries cows for better adaptation in subtropical and tropical hot climate.