Effect of iron stress on Withania somnifera L.: antioxidant enzyme response and nutrient elemental uptake of in vitro grown plants
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- 作者：Jyoti Ranjan Rout ; Sadhana Behera ; Nitin Keshari ; Shidharth Sankar Ram…
- 关键词：Antioxidant enzymes ; Bioaccumulation ; Gene expression ; Iron stress ; Proton induced X ; ray emission (PIXE) ; Withania somnifera L.
- 刊名：Ecotoxicology
- 出版年：2015
- 出版时间：March 2015
- 年：2015
- 卷：24
- 期：2
- 页码：401-413
- 全文大小：855 KB
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19. Kwon SI, Lee H, An CS (2007) Differential expression of three catalase genes in the small radish ( / Rhphanus sativus L. v - 作者单位：Jyoti Ranjan Rout (1) (4)
Sadhana Behera (2)
Nitin Keshari (2)
Shidharth Sankar Ram (3)
Subhajit Bhar (3)
Anindita Chakraborty (3)
Mathummal Sudarshan (3)
Santi Lata Sahoo (1)
1. Biochemistry and Molecular Biology Laboratory, Post Graduate Department of Botany, Utkal University, Vani Vihar, Bhubaneswar, 751004, Odisha, India
4. Post Graduate Department of Biotechnology, Academy of Management and Information Technology, 67/68, IID Centre, Bidya Vihar, Barunei Hills, Khurda, 752057, Odisha, India
2. Department of Biotechnology, Utkal University, Vani Vihar, Bhubaneswar, 751004, Odisha, India
3. UGC-DAE Consortium of Scientific Research, Kolkata Centre, Bidhan Nagar, Salt Lake, Kolkata, 700098, India - 刊物类别：Earth and Environmental Science
- 刊物主题：Environment
Environment
Monitoring, Environmental Analysis and Environmental Ecotoxicology
Ecology
Environmental Management - 出版者：Springer Netherlands
- ISSN：1573-3017
文摘
In the present study the response of antioxidant enzyme activities and the level of expression of their corresponding genes on bioaccumulation of iron (Fe) were investigated. In vitro germinated Withania somnifera L. were grown in Murashige and Skoog’s liquid medium with increasing concentrations (0, 25, 50, 100 and 200?μM) of FeSO4 for 7 and 14?days. Root and leaf tissues analyzed for catalase (CAT, EC 1.11.1.6), superoxide dismutase (SOD, EC 1.15.1.1) and guaiacol peroxidase (GPX, EC 1.11.1.7), have shown an increase in content with respect to exposure time. Isoforms of CAT, SOD and GPX were separated using non-denaturing polyacrylamide gel electrophoresis and observed that the isoenzymes were greatly affected by higher concentrations of Fe. Reverse transcriptase polymerase chain reaction analysis performed by taking three pairs of genes of CAT (RsCat, Catalase1, Cat1) and SOD (SodCp, TaSOD1.2, MnSOD) to find out the differential expression of antioxidant genes under Fe excess. RsCat from CAT and MnSOD from SOD have exhibited high levels of gene expression under Fe stress, which was consistent with the changes of the activity assayed in solution after 7?days of treatment. Analysis by proton induced X-ray emission exhibited an increasing uptake of Fe in plants by suppressing and expressing of other nutrient elements. The results of the present study suggest that higher concentration of Fe causes disturbance in nutrient balance and induces oxidative stress in plant.