文摘
Quantitative estimation of inorganic elements of cotyledon-derived callus tissues of a medicinal legume, Abrus precatorius L. was determined using Proton induced X-ray emission technique. Nine trace elements namely Cu, Cr, Co, Zn, Mn, Fe, Se, Br and Ti in addition to two macro-elements K and Ca were identified, quantified and compared with each culture passage to monitor the variation in trace element accumulation. Experimental findings revealed that, 10-week-old calli was more efficient than others in accumulating inorganic elements. These results through light on the implication of in vitro callus cultures as a viable, alternative and proliferating renewable resource of medicinally useful elements for designing of drugs and also helping in natural germplasm conservation.