Effects of Focal Cerebral Ischemia on Exosomal Versus Serum miR126

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• 作者：Fan Chen ; Yang Du ; Elga Esposito ; Yi Liu ; Shuzhen Guo…
• 关键词：Focal cerebral ischemia ; miR ; 126 ; Exosome ; Biomarker ; Reperfusion injury
• 刊名：Translational Stroke Research
• 出版年：2015
• 出版时间：December 2015
• 年：2015
• 卷：6
• 期：6
• 页码：478-484
• 全文大小：425 KB
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• 作者单位：Fan Chen (1) (2)
  Yang Du (2) (3)
  Elga Esposito (2)
  Yi Liu (1) (2)
  Shuzhen Guo (2)
  Xiaoying Wang (2)
  Eng H. Lo (2)
  Changhong Xing (2)
  Xunming Ji (1)
  
  1. Cerebrovascular Diseases Research Institute, Xuanwu Hospital of Capital Medical University, Beijing, China
  2. Massachusetts General Hospital, Harvard Medical School, Boston, USA
  3. Department of Neurology, Beijing Tiantan Hospital, Capital Medical University, Beijing, China
  
• 刊物主题：Neurosciences; Neurology; Cardiology; Neurosurgery; Vascular Surgery;
• 出版者：Springer US
• ISSN：1868-601X

文摘

Emerging data suggest that exosomal microRNA (miRNA) may provide potential biomarkers in acute ischemic stroke. However, the effects of ischemia-reperfusion on total versus exosomal miRNA responses in circulating blood remain to be fully defined. Here, we quantified levels of miR-126 in whole serum versus exosomes extracted from serum and compared these temporal profiles against reperfusion and outcomes in a rat model of acute focal cerebral ischemia. First, in vitro experiments confirmed the vascular origin and changes in miR-126 in brain endothelial cultures subjected to oxygen-glucose deprivation. Then in vivo experiments were performed by inducing permanent or transient focal cerebral ischemia in rats, and total serum and exosomal miR-126 levels were quantified, along with measurements of infarction and neurological outcomes. Exosomal levels of miR-126 showed a transient reduction at 3 h post-ischemia that appeared to normalize back close to pre-ischemic baselines after 24 h. There were no detectable differences in exosomal miR-126 responses in permanent or transient ischemia. Serum miR-126 levels appeared to differ in permanent versus transient ischemia. Significant reductions in serum miR-126 were detected at 3 h after permanent ischemia but not transient ischemia. By 24 h, serum miR-126 levels were back close to baseline in both permanent and transient ischemia. Overall, there were no correlations between serum miR-126 and exosomal miR-126. This proof-of-concept study suggests that changes in serum miR-126 may be able to distinguish severe permanent ischemia from milder injury after transient ischemia. Keywords Focal cerebral ischemia miR-126 Exosome Biomarker Reperfusion injury