Experimental infection with cytopathic bovine viral diarrhea virus in mice induces megakaryopoiesis in the spleen and bone marrow

详细信息    查看全文

• 作者：Giyong Seong ; Jin-Sol Lee ; Kyung-Hyun Lee ; Kyoung-Seong Choi
• 刊名：Archives of Virology
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：161
• 期：2
• 页码：417-424
• 全文大小：3,971 KB
• 参考文献：1.Zhang G, Aldridge S, Clarke MC, McCauley JW (1996) Cell death induced by cytopathic bovine viral diarrhoea virus is mediated by apoptosis. J Gen Virol 77:1677–1681CrossRef PubMed
  2.Baker JC (1995) The clinical manifestations of bovine viral diarrhea infection. Vet Clin N Am Food Anim Pract 11:425–445CrossRef
  3.Ridpath JF, Bendfeldt S, Neill JD, Liebler-Tenorio E (2006) Lymphocytopathogenic activity in vitro correlates with high virulence in vivo for BVDV type 2 strains: Criteria for a third biotype of BVDV. Virus Res 118:62–69CrossRef PubMed
  4.Dubovi EJ (1994) Impact of bovine viral diarrhea virus on reproductive performance in cattle. Vet Clin N Am Food Anim Pract 10:503–514
  5.Brownlie J, Clarke MC, Howard CJ (1984) Experimental production of fatal mucosal disease in cattle. Vet Rec 114:535–536CrossRef PubMed
  6.Seong G, Oem JK, Choi KS (2015) The first finding of the ophthalmosteresis in a Korean native calf with bovine viral diarrhea virus. Vet Arhiv 85:111–116
  7.Corapi WV, Elliott RD, French TW, Arthur DG, Bezek DM, Dubovi EJ (1990) Thrombocytopenia and hemorrhages in veal calves infected with bovine viral diarrhea virus. J Am Vet Med Assoc 196:590–596PubMed
  8.Spagnuolo M, Kennedy S, Foster JC, Moffett DA, Adair BM (1997) Bovine viral diarrhoea virus infection in bone marrow of experimentally infected calves. J Comp Pathol 116:97–100CrossRef PubMed
  9.Wood RD, Goens SD, Carman PS, Deregt D, Jefferson B, Jacobs RM (2004) Effect on hematopoietic tissue of experimental infection of calves with noncytopathic type 2 bovine viral diarrhea virus. Can J Vet Res 68:42–48PubMedCentral PubMed
  10.Walz PH, Bell TG, Steficek BA, Kaiser L, Maes RK, Baker JC (1999) Experimental model of type II bovine viral diarrhea virus-induced thrombocytopenia in neonatal calves. J Vet Diagn Invest 11:505–514CrossRef PubMed
  11.Corapi WV, French TW, Dubovi EJ (1989) Severe thrombocytopenia in young calves experimentally infected with noncytopathic bovine viral diarrhea virus. J Virol 63:3934–3943PubMedCentral PubMed
  12.Marshall D, Moxley RA, Kelling CL (1996) Distribution of virus and viral antigen in specific pathogen-free calves following inoculation with noncytopathic bovine viral diarrhea virus. Vet Pathol 33:311–318CrossRef PubMed
  13.Gómez-Villamandos JC, Ruiz-Villamor E, Salguero FJ, Bautista MJ, Carrasco L, Sánchez C, Quezada M, Sierra MA (1998) Immunohistochemical and ultrastructural evidence of hog cholera virus infection of megakaryocytes in bone marrow and spleen. J Comp Pathol 119:111–119CrossRef PubMed
  14.Vilcek S, Herring AJ, Herring JA, Nettleton PF, Lowings JP, Paton DJ (1994) Pestiviruses isolated from pigs, cattle and sheep can be allocated into at least three genogroups using polymerase chain reaction and restriction endonuclease analysis. Arch Virol 136:309–323CrossRef PubMed
  15.Seong G, Oem JK, Lee JH, Choi KS (2015) Experimental infection of mice with bovine viral diarrhea virus. Arch Virol 160:1565–1571CrossRef PubMed
  16.Seong G, Oem JK, Choi KS (2013) Pathogenetic differences after experimental infection of calves with Korean non-cytopathic BVDV-1 and BVDV-2 isolates. Vet Immunol Immunopathol 156:147–152CrossRef PubMed
  
• 作者单位：Giyong Seong (1)
  Jin-Sol Lee (1)
  Kyung-Hyun Lee (2)
  Kyoung-Seong Choi (1)
  
  1. College of Ecology and Environmental Science, Kyungpook National University, Sangju, 742-711, Republic of Korea
  2. Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency, Anyang, 430-824, Republic of Korea
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine
  Virology
  Medical Microbiology
  Infectious Diseases
  
• 出版者：Springer Wien
• ISSN：1432-8798

文摘

Here, we infected mice with cytopathic bovine viral diarrhea virus 1 (cp BVDV1) by oral inoculation and investigated the effects of infection by histopathological, immunohistochemical (IHC), hematological methods. Twelve mice were infected, and samples were obtained at day 2, 5, and 9 postinfection (pi). Most of the infected mice exhibited clinical signs of illness such as reduced movement, crouching, loose feces, loss of appetite, and reduced water intake. Blood samples from six mice were positive for BVDV based on reverse transcription polymerase chain reaction (RT-PCR). Blood analysis also revealed thrombocytopenia and lymphopenia. Viral antigens were detected in the spleen (12/12), bone marrow (12/12), and/or mesenteric lymph nodes (4/12) of all infected mice by IHC analysis. The spleens showed significant histopathological changes including (i) substantially increased numbers of megakaryocytes, (ii) lymphocyte depletion, and (iii) hemorrhages. The bone marrow also had an increased number of megakaryocytes, although this increase was not as strong as it was in the spleen. Severe lymphoid depletion was observed in the mesenteric lymph nodes. Viral infections were present in the lymphocytes but not detected in megakaryocytes of the spleen, bone marrow, or mesenteric lymph nodes. These results suggest that the increased numbers of megakaryocytes may be a direct result of BVDV infection. BVDV infection in mice following oral inoculation of cp BVDV1 leads to megakaryopoiesis in the spleen and bone marrow to replenish the platelets. G. Seong, J.-S. Lee and K.-H. Lee contributed equally to this work.