Development and validation of the modular Feed-code method for qualitative and quantitative determination of feed botanical composition

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• 作者：Luca Braglia ; Silvia Gianì ; Diego Breviario…
• 关键词：Feed ; Traceability ; Intron ; TBP ; Feed ; code ; qPCR
• 刊名：Analytical and Bioanalytical Chemistry
• 出版年：2016
• 出版时间：November 2016
• 年：2016
• 卷：408
• 期：29
• 页码：8299-8316
• 全文大小：1,438 KB
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Analytical Chemistry
  Food Science
  Inorganic Chemistry
  Physical Chemistry
  Monitoring, Environmental Analysis and Environmental Ecotoxicology
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1618-2650
• 卷排序：408

文摘

The analysis of feed composition in terms of ingredients is addressed by Regulation (EC) 767/2009 and is important for detecting economic fraud and for monitoring feed safety. Within the framework of the EU project Feed-code, we developed and internally validated a modular assay, relying on intron polymorphism, for the complete qualitative analysis of the botanical composition of feed and the quantitative determination of six target plant species. Main performance parameters of each module, such as applicability, repeatability, specificity, and limit of detection, were evaluated. The whole assay was applied to a set of feed-like samples and results were in agreement with the expected composition. Application to a large set of compound feed and individual raw materials revealed the occurrence of botanical impurities. When compared with microscopic analysis, the proposed method gave more reliable results. We conclude that the Feed-code prototype, readily upgradable to include more plant species, is worthy of consideration for a full validation through a collaborative trial.