Visualisation of plastid degradation in sperm cells of wheat pollen
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- 作者：Lucia F. Primavesi ; Huixia Wu ; Elisabeth A. Mudd ; Anil Day ; Huw D. Jones
- 关键词：Wheat ; Pollen ; GFP ; Plastid ; Sperm cell ; Plastid networks ; Maternal inheritance
- 刊名：Protoplasma
- 出版年：2017
- 出版时间：January 2017
- 年：2017
- 卷：254
- 期：1
- 页码：229-237
- 全文大小：
- 刊物类别：Biomedical and Life Sciences
- 刊物主题：Cell Biology; Plant Sciences; Zoology;
- 出版者：Springer Vienna
- ISSN：1615-6102
- 卷排序：254
文摘
Like most angiosperms, wheat (Triticum aestivum) shows maternal inheritance of plastids. It is thought that this takes place by cytoplasmic stripping at fertilisation rather than the absence of plastids in sperm cells. To determine the fate of plastids during sperm cell development, plastid-targeted green fluorescent protein was used to visualise these organelles in nuclear transgenic wheat lines. Fewer than thirty small 1–2-μm plastids were visible in early uninucleate pollen cells. These dramatically increased to several hundred larger (4 μm) plastids during pollen maturation and went through distinct morphological changes. Only small plastids were visible in generative cells (n = 25) and young sperm cells (n = 9). In mature sperm cells, these green fluorescent protein (GFP)-tagged plastids were absent. This is consistent with maternal inheritance of plastids resulting from their degradation in mature sperm cells in wheat.