Activation of 5-HT2A/C receptor reduces glycine receptor-mediated currents in cultured auditory cortical neurons
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- 作者：Bin Luo ; Lingli Hu ; Chunhua Liu ; Yiping Guo ; Haitao Wang
- 关键词：5 ; HT2A/C receptors ; Glycine receptors ; Patch clamp ; Auditory cortex
- 刊名：Amino Acids
- 出版年：2016
- 出版时间：February 2016
- 年：2016
- 卷：48
- 期：2
- 页码：349-356
- 全文大小：991 KB
- 参考文献：Andrade R (1998) Regulation of membrane excitability in the central nervous system by serotonin receptor subtypes. Ann N Y Acad Sci 861:190–203CrossRef PubMed
Barnes NM, Sharp T (1999) A review of central 5-HT receptors and their function. Neuropharmacology 38:1083–1152CrossRef PubMed
Benazzi F, Berk M, Frye MA, Wang W, Barraco A, Tohen M (2009) Olanzapine/fluoxetine combination for the treatment of mixed depression in bipolar I disorder: a post hoc analysis. J Clin Psychiatry 70:1424–1431CrossRef PubMed
Benekareddy M, Goodfellow NM, Lambe EK, Vaidya VA (2010) Enhanced function of prefrontal serotonin 5-HT(2) receptors in a rat model of psychiatric vulnerability. J Neurosci 30:12138–12150PubMedCentral CrossRef PubMed
Boyer EW, Shannon M (2005) The serotonin syndrome. N Engl J Med 352:1112–1120CrossRef PubMed
Charrier C, Ehrensperger MV, Dahan M, Levi S, Triller A (2006) Cytoskeleton regulation of glycine receptor number at synapses and diffusion in the plasma membrane. J Neurosci 26:8502–8511CrossRef PubMed
Ciranna L (2006) Serotonin as a modulator of glutamate- and GABA-mediated neurotransmission: implications in physiological functions and in pathology. Curr Neuropharmacol 4:101–114PubMedCentral CrossRef PubMed
Deng PY, Lei S (2008) Serotonin increases GABA release in rat entorhinal cortex by inhibiting interneuron TASK-3 K+ channels. Mol Cell Neurosci 39:273–284PubMedCentral CrossRef PubMed
Doya K (2002) Metalearning and neuromodulation. Neural Netw 15:495–506CrossRef PubMed
Eggermont JJ, Roberts LE (2004) The neuroscience of tinnitus. Trends Neurosci 27:676–682CrossRef PubMed
Farrant M, Nusser Z (2005) Variations on an inhibitory theme: phasic and tonic activation of GABA(A) receptors. Nat Rev Neurosci 6:215–229CrossRef PubMed
Feng J, Cai X, Zhao J, Yan Z (2001) Serotonin receptors modulate GABA(A) receptor channels through activation of anchored protein kinase C in prefrontal cortical neurons. J Neurosci 21:6502–6511PubMed
Komlosi G, Molnar G, Rozsa M, Olah S, Barzo P, Tamas G (2012) Fluoxetine (prozac) and serotonin act on excitatory synaptic transmission to suppress single layer 2/3 pyramidal neuron-triggered cell assemblies in the human prefrontal cortex. J Neurosci 32:16369–16378PubMedCentral CrossRef PubMed
Lund JS, Angelucci A, Bressloff PC (2003) Anatomical substrates for functional columns in macaque monkey primary visual cortex. Cereb Cortex 13:15–24CrossRef PubMed
Maguire EP et al (2014) Extrasynaptic glycine receptors of rodent dorsal raphe serotonergic neurons: a sensitive target for ethanol. Neuropsychopharmacology 39:1232–1244PubMedCentral CrossRef PubMed
Manzke T et al (2010) Serotonin receptor 1A-modulated phosphorylation of glycine receptor alpha3 controls breathing in mice. J Clin Invest 120:4118–4128PubMedCentral CrossRef PubMed
Moreau AW, Amar M, Le Roux N, Morel N, Fossier P (2010) Serotoninergic fine-tuning of the excitation-inhibition balance in rat visual cortical networks. Cereb Cortex 20:456–467CrossRef PubMed
Salling MC, Harrison NL (2014) Strychnine-sensitive glycine receptors on pyramidal neurons in layers II/III of the mouse prefrontal cortex are tonically activated. J Neurophysiol 112:1169–1178PubMedCentral CrossRef PubMed
Specht CG, Grunewald N, Pascual O, Rostgaard N, Schwarz G, Triller A (2011) Regulation of glycine receptor diffusion properties and gephyrin interactions by protein kinase C. EMBO J 30:3842–3853PubMedCentral CrossRef PubMed
Tang ZQ, Lu YG, Chen L (2008) Developmental stability of taurine’s activation on glycine receptors in cultured neurons of rat auditory cortex. Neurosci Lett 430:54–59CrossRef PubMed
Vertes RP, Kocsis B (1994) Projections of the dorsal raphe nucleus to the brainstem: PHA-L analysis in the rat. J Comp Neurol 340:11–26CrossRef PubMed
Wang HT, Luo B, Huang YN, Zhou KQ, Chen L (2008) Sodium salicylate suppresses serotonin-induced enhancement of GABAergic spontaneous inhibitory postsynaptic currents in rat inferior colliculus in vitro. Hear Res 236:42–51CrossRef PubMed
Wang H, Han YF, Chan YS, He J (2014) Stimulus-specific adaptation at the synapse level in vitro. Plos One 9:e114537PubMedCentral CrossRef PubMed
Wilson MA, Molliver ME (1991) The organization of serotonergic projections to cerebral cortex in primates: retrograde transport studies. Neuroscience 44:555–570CrossRef PubMed
Xie DJ, Uta D, Feng PY, Wakita M, Shin MC, Furue H, Yoshimura M (2012) Identification of 5-HT receptor subtypes enhancing inhibitory transmission in the rat spinal dorsal horn in vitro. Mol Pain 8:58PubMedCentral CrossRef PubMed
Xu H, Wang W, Tang ZQ, Xu TL, Chen L (2006) Taurine acts as a glycine receptor agonist in slices of rat inferior colliculus. Hear Res 220:95–105CrossRef PubMed
Yang S, Weiner BD, Zhang LS, Cho SJ, Bao S (2011) Homeostatic plasticity drives tinnitus perception in an animal model. Proc Natl Acad Sci USA 108:14974–14979PubMedCentral CrossRef PubMed
Yuen EY, Jiang Q, Chen P, Feng J, Yan Z (2008) Activation of 5-HT2A/C receptors counteracts 5-HT1A regulation of n-methyl-D-aspartate receptor channels in pyramidal neurons of prefrontal cortex. J Biol Chem 283:17194–17204PubMedCentral CrossRef PubMed
Zhou FM, Hablitz JJ (1999) Activation of serotonin receptors modulates synaptic transmission in rat cerebral cortex. J Neurophysiol 82:2989–2999PubMed - 作者单位：Bin Luo (1)
Lingli Hu (2) (3)
Chunhua Liu (2) (3)
Yiping Guo (2) (3)
Haitao Wang (2) (3)
1. Department of Otolaryngology, Anhui Provincial Hospital Affiliated to Anhui Medical University, Hefei, 230001, China
2. Key Laboratory of Regenerative Biology, Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, 510530, Guangdong, China
3. Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangzhou, 510530, Guangdong, China - 刊物类别：Biomedical and Life Sciences
- 刊物主题：Life Sciences
Biochemistry
Analytical Chemistry
Biochemical Engineering
Life Sciences
Proteomics
Neurobiology - 出版者：Springer Wien
- ISSN：1438-2199
文摘
Glycine receptors (GlyRs) permeable to chloride only mediate tonic inhibition in the cerebral cortex where glycinergic projection is completely absent. The functional modulation of GlyRs was largely studied in subcortical brain regions with glycinergic transmissions, but the function of cortical GlyRs was rarely addressed. Serotonin could broadly modulate many ion channels through activating 5-HT2 receptor, but whether cortical GlyRs are subjected to serotonergic modulation remains unexplored. The present study adopted patch clamp recordings to examine functional regulation of strychnine-sensitive GlyRs currents in cultured cortical neurons by DOI (2,5-Dimethoxy-4-iodoamphetamine), a 5-HT2A/C receptor agonist. DOI caused a concentration-dependent reduction of GlyR currents with unchanged reversal potential. This reduction was blocked by the selective receptor antagonists (ritanserin and risperidone) and G protein inhibitor (GDP-β-s) demonstrated that the reducing effect of DOI on GlyR current required the activation of 5-HT2A/C receptors. Strychnine-sensitive tonic currents revealed the inhibitory tone mediated by nonsynaptic GlyRs, and DOI similarly reduced the tonic inhibition. The impaired microtube-dependent trafficking or clustering of GlyRs was thought to be involved in that nocodazole as a microtube depolymerizing drug largely blocked the inhibition mediated by 5-HT2A/C receptors. Our results suggested that activation of 5-HT2A/C receptors might suppress cortical tonic inhibition mediated by GlyRs, and the findings would provide important insight into serotonergic modulation of tonic inhibition mediated by GlyRs, and possibly facilitate to develop the therapeutic treatment of neurological diseases such as tinnitus through regulating cortical GlyRs.