Genome edited sheep and cattle

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• 作者：Chris Proudfoot (1)
  Daniel F. Carlson (2)
  Rachel Huddart (1)
  Charles R. Long (3)
  Jane H. Pryor (3)
  Tim J. King (1)
  Simon G. Lillico (1)
  Alan J. Mileham (4)
  David G. McLaren (4)
  C. Bruce A. Whitelaw (1)
  Scott C. Fahrenkrug (2)
  
• 关键词：Livestock ; TALEN ; Myostatin ; Zygote ; Genetic engineering
• 刊名：Transgenic Research
• 出版年：2015
• 出版时间：February 2015
• 年：2015
• 卷：24
• 期：1
• 页码：147-153
• 全文大小：768 KB
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• 作者单位：Chris Proudfoot (1)
  Daniel F. Carlson (2)
  Rachel Huddart (1)
  Charles R. Long (3)
  Jane H. Pryor (3)
  Tim J. King (1)
  Simon G. Lillico (1)
  Alan J. Mileham (4)
  David G. McLaren (4)
  C. Bruce A. Whitelaw (1)
  Scott C. Fahrenkrug (2)
  
  1. The Roslin Institute and R(D)SVS, University of Edinburgh, Easter Bush Campus, Edinburgh, EH25 9RG, UK
  2. Recombinetics Inc, 2575 University Ave. West, Suite 100, Saint Paul, MN, 55108, USA
  3. Department of Veterinary Physiology and Pharmacology, College of Veterinary Medicine, Texas A&M University, College Station, TX, 77843, USA
  4. Genus plc, 1525 River Road, DeForest, WI, 53532, USA
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine
  Molecular Medicine
  Plant Genetics and Genomics
  Animal Genetics and Genomics
  Plant Sciences
  Human Genetics
  
• 出版者：Springer Netherlands
• ISSN：1573-9368

文摘

Genome editing tools enable efficient and accurate genome manipulation. An enhanced ability to modify the genomes of livestock species could be utilized to improve disease resistance, productivity or breeding capability as well as the generation of new biomedical models. To date, with respect to the direct injection of genome editor mRNA into livestock zygotes, this technology has been limited to the generation of pigs with edited genomes. To capture the far-reaching applications of gene-editing, from disease modelling to agricultural improvement, the technology must be easily applied to a number of species using a variety of approaches. In this study, we demonstrate zygote injection of TALEN mRNA can also produce gene-edited cattle and sheep. In both species we have targeted the myostatin (MSTN) gene. In addition, we report a critical innovation for application of gene-editing to the cattle industry whereby gene-edited calves can be produced with specified genetics by ovum pickup, in vitro fertilization and zygote microinjection (OPU-IVF-ZM). This provides a practical alternative to somatic cell nuclear transfer for gene knockout or introgression of desirable alleles into a target breed/genetic line.