Substance-P blocks degeneration of retina by stimulating migration and proliferation of retinal pigmented epithelial cells

详细信息    查看全文

• 作者：Hyun Sook Hong (1)
  Suna Kim (2)
  Yeong Hoon Kim (3)
  Ju Hyeong Park (2)
  Yinji Jin (2)
  Youngsook Son (2)
  
  1. College of Medicine/ East-West Medical Research Institute ; Kyung Hee University ; 1 Hoegi-dong. Dongdaemun-gu ; Seoul ; 130-702 ; Korea
  2. Department of Genetic Engineering ; College of Life Science and Graduate School of Biotechnology ; Kyung Hee University ; Seochundong ; Kiheung-ku ; Yong In ; 441-706 ; Korea
  3. Department of Ophthalmology ; College of Medicine ; The Catholic University of Korea ; St. Paul鈥檚 Hospital ; Wangsan street ; Dongdaemun-gu Seoul ; 130-709 ; Korea
  
• 关键词：Substance ; P ; retinal pigment epithelium ; proliferation ; migration
• 刊名：Tissue Engineering and Regenerative Medicine
• 出版年：2015
• 出版时间：April 2015
• 年：2015
• 卷：12
• 期：2
• 页码：121-127
• 全文大小：1,562 KB
• 参考文献：1. Klein, B E, Klein, R (1982) Cataracts and macular degeneration in older Americans. Arch. Ophthalmol 100: pp. 571-573 CrossRef
  Vision Research: Anational Plan. Executive Summary, 1983鈥?987.
  2. Harris, JR, Brown, GA, Jorgensen, M, Kaushal, S, Ellis, EA, Grant, MB (2006) Bone marrow-derived cells home to and regenerate retinal pigment epithelium after injury. Invest Ophthalmol Vis Sci 47: pp. 2108-2113 CrossRef
  3. Binder, S, Stanzel, BV, Krebs, I, Glittenberg, C (2007) Transplantation of the RPE in AMD. Prog Retin Eye Res 26: pp. 516-554 CrossRef
  4. Toma, HS, Barnett, JM, Penn, JS, Kim, SJ (2010) Improved assessment of laser-induced choroidal neovascularization. Microvasc Res 80: pp. 295-302 CrossRef
  5. Machemer, R, Steinhorst, U (1993) Retinal separation, retinotomy, and macular relocation. A surgical approach for age-related macular degeneration. Graefes Arch. Clin. Exp. Ophthalmol 231: pp. 635-641 CrossRef
  6. Avitabile, T, Longo, A, Reibaldi, A (2005) Intravitreal triamcinolone compared with macular laser grid photocoagulation for the treatment of cystoid macular edema. Am J Ophthalmol 140: pp. 695-702 CrossRef
  7. Er, H, Yilmaz, H (2005) Intravitreal cortisone injection for refractory diffuse diabetic macular edema. Ophthalmologica 219: pp. 394-400 CrossRef
  8. Jonas, JB, Akkoyun, I, Kreissig, I, Degenring, RF (2005) Diffuse diabetic macular edema treated by intravitreal triamcinolone acetonide: a comparative, non-randomised study. Br J Ophthalmol 89: pp. 321-326 4.046391" target="_blank" title="It opens in new window">CrossRef
  9. Hong, H S, Lee, J, Lee, E, Kwon, Y S, Lee, E, Ahn, W, Jiang, M H, Kim, J C, Son, Y (2009) A new role of substance P as an injury-inducible messenger for mobilization of CD29(+) stromal-like cells. Nat. Med 15: pp. 425-435 CrossRef
  10. Mantyh, PW (2002) Neurobiology of substance P and the NK1 receptor. J Clin Psychiatry 11: pp. 6-10
  11. Bossaller, C, Reither, K, Hehlert-Friedrich, C, Auch-Schwelk, W, Graf, K, Gr盲fe, M, Fleck, E (1992) In vivo measurement of endothelium-dependent vasodilation with substance P in man. Herz 175: pp. 284-90
  12. Rameshwar, P (2001) The dynamics of bone marrow stromal cells in the proliferation of multipotent hematopoietic progenitors by substance P: an understanding of the effects of neurotransmitter on the differentiating hematopoietic stem cell. J. Neuroimmuno 121: pp. 22-31 443-X" target="_blank" title="It opens in new window">CrossRef
  13. Nakamura, M (2003) Promotion of corneal epithelial wound healing in diabetic rats by the combination of a substance P鈥揹erived peptide (FGLM-NH2) and insulin-like growth factor-1. Diabetologia 46: pp. 839-842 CrossRef
  14. Yang, CM (2002) Substance P-induced activation of p42/44 mitogen-activated protein kinase associated with cell proliferation in human tracheal smooth muscle cells. Cellular Signaling 14: pp. 913-923 CrossRef
  15. Reid, TW, Murphy, CJ, Iwahashi, CK, Foster, BA, Mannis, MJ (1993) Stimulation of epithelial cell growth by the neuropeptide substance P. Journal of Cellular Biochemistry 52: pp. 476-85 40520411" target="_blank" title="It opens in new window">CrossRef
  16. Sheedlo, HJ, Li, L, Fan, W, Turner, JE (1995) Retinal pigment epithelial cell support of photoreceptor survival in vitro. In Vitro Cell Dev Biol Anim. 31: pp. 330-3 4278" target="_blank" title="It opens in new window">CrossRef
  17. Vingerling, JR, Dielemans, I, Hofman, A (1995) The prevalence of age-related maculopathy in the Rotterdam Study. Ophthalmology 102: pp. 205-210 420(95)31034-2" target="_blank" title="It opens in new window">CrossRef
  18. Klein, BE, Klein, R, Linton, KL (1992) Prevalence of age-related lens opacities in a population, The Beaver Dam Eye Study. Ophthalmology 99: pp. 546-552 420(92)31934-7" target="_blank" title="It opens in new window">CrossRef
  
• 刊物主题：Biomedicine general; Biomedical Engineering; Cell Biology;
• 出版者：Springer Netherlands
• ISSN：2212-5469

文摘

The retinal pigment epithelium (RPE) is a monolayer to function as a compact barrier between photoreceptor and choroid and also a regulator of the overlying photoreceptor layer by providing nutrients. If RPE is damaged, choroidal neovascularization is occurred, accompanied by inflammation, which leads to degeneration of whole retina layer and furthermore, loss of vision. Thus, the preservation of RPE layer is very critical treatment to block the progression and occurrence of diverse ocular disease, inducing age-related macular degeneration (AMD). In this study, we explored the new role of SP in RPE regeneration. After confirming the expression of NK-1R on ARPE-19 cells, we treated SP to ARPE-19 cells in vitro to evaluate the effect of SP on proliferation rate and migratory capacity. SP could promote proliferation and migration of ARPE-19 in dose-dependent manner in vitro. Based on the in vitro data, 5nmole/kg of SP was intravitreally treated to laser-induced RPE damaged mice in vivo, showing that PBS-injected mice had severed disturbed retina layer, whereas SP-injected mice maintained retina layer more intact without detachment of RPE. In addition to histological comparison, the efficacy of SP was assessed according to the score that indicate the severity of retina degeneration. This confirmed that SP can exert the beneficial effect on laser-induced RPE damage. Collectively, our findings suggest that SP can stimulate the regeneration of RPE layer and thus, it may be possible that SP is used as the new therapeutics for RPE-damaged disease including AMD.