A mouse line for inducible and reversible silencing of specific neurons

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• 作者：Ling Hu (1) (2) (3)
  Wei Lan (1) (2) (3)
  Hao Guo (4)
  Guo-Dong Chai (5)
  Kun Huang (5)
  Ling Zhang (1) (2)
  Ying Huang (1) (2)
  Xue-Feng Chen (4)
  Lei Zhang (1) (2)
  Ning-Ning Song (1) (2)
  Ling Chen (1) (2)
  Bing Lang (3)
  Yun Wang (6)
  Qing-Xiu Wang (5)
  Jin-Bao Zhang (7)
  Collin McCaig (3)
  Lin Xu (4)
  Yu-Qiang Ding (1) (2)
  
  1. Key Laboratory of Arrhythmias ; Ministry of Education of China ; East Hospital ; Tongji University School of Medicine ; Shanghai ; 200120 ; China
  2. Department of Anatomy and Neurobiology ; Tongji University School of Medicine ; Shanghai ; 200092 ; China
  3. School of Medical Sciences ; Institute of Medical Sciences ; University of Aberdeen ; Foresterhill ; Aberdeen ; AB25 2ZD ; UK
  4. Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences and Yunnan Province ; Kunming Institute of Zoology ; Kunming ; Yunnan ; 650223 ; China
  5. Department of Anesthesiology ; East Hospital ; Tongji University School of Medicine ; Shanghai ; 200120 ; China
  6. Institutes of Brain Science ; State Key Laboratory for Medical Neurobiology ; Fudan University ; Shanghai ; 200032 ; China
  7. Institute of Neuroscience ; School of Basic Medical Sciences ; Wenzhou Medical University ; Wenzhou ; Zhejiang ; 325035 ; China
  
• 关键词：Neuron silencing ; Ligand ; gated channel ; Ivermectin ; Rosa26
• 刊名：Molecular Brain
• 出版年：2014
• 出版时间：December 2014
• 年：2014
• 卷：7
• 期：1
• 全文大小：1,916 KB
• 参考文献：1. Luo, L, Callaway, EM, Svoboda, K (2008) Genetic dissection of neural circuits. Neuron 57: pp. 634-660 CrossRef
  2. Miesenb枚ck, G (2004) Genetic methods for illuminating the function of neural circuits. Curr Opin Neurobiol 14: pp. 395-402 4.05.004" target="_blank" title="It opens in new window">CrossRef
  3. Callaway, EM (2005) A molecular and genetic arsenal for systems neuroscience. Trends Neurosci 28: pp. 196-201 CrossRef
  4. Karpova, AY, Tervo, DGR, Gray, NW, Svoboda, K (2005) Rapid and reversible chemical inactivation of synaptic transmission in genetically targeted neurons. Neuron 48: pp. 727-735 CrossRef
  5. Birg眉l, N, Weise, C, Kreienkamp, HJ, Richter, D (1999) Reverse physiology in Drosophila: identification of a novel allatostatin-like neuropeptide and its cognate receptor structurally related to the mammalian somatostatin/galanin/opioid receptor family. EMBO J 18: pp. 5892-5900 CrossRef
  6. Lechner, HAE, Lein, ES, Callaway, EM (2002) A genetic method for selective and quickly reversible silencing of mammalian neurons. J Neurosci 22: pp. 5287-5290
  7. Tan, EM, Yamaguchi, Y, Horwitz, GD, Gosgnach, S, Lein, ES, Goulding, M, Albright, TD, Callaway, EM (2006) Selective and quickly reversible inactivation of mammalian neurons in vivo using the Drosophila allatostatin receptor. Neuron 51: pp. 157-170 CrossRef
  8. Gosgnach, S, Lanuza, GM, Butt, SJB, Saueressig, H, Zhang, Y, Velasquez, T, Riethmacher, D, Callaway, EM, Kiehn, O, Goulding, M (2006) V1 spinal neurons regulate the speed of vertebrate locomotor outputs. Nature 440: pp. 215-219 4545" target="_blank" title="It opens in new window">CrossRef
  9. Wulff, P, Goetz, T, Leppa, E, Linden, A-M, Renzi, M, Swinny, JD, Vekovischeva, OY, Sieghart, W, Somogyi, P, Korpi, ER, Farrant, M, Wisden, W (2007) From synapse to behavior: rapid modulation of defined neuronal types with engineered GABAA receptors. Nat Neurosci 10: pp. 923-929 CrossRef
  10. Cully, DF, Vassilatis, DK, Liu, KK, Paress, PS, Van der Ploeg, LHT, Schaeffer, JM, Arena, JP (1994) Cloning of an avermectin-sensitive glutamate-gated chloride channel from Caenorhabditis elegans. Nature 371: pp. 707-711 CrossRef
  11. Lerchner, W, Xiao, C, Nashmi, R, Slimko, EM, Van Trigt, L, Lester, HA, Anderson David, J (2007) Reversible silencing of neuronal excitability in behaving mice by a genetically targeted, ivermectin-gated Cl鈥夆垝鈥塩hannel. Neuron 54: pp. 35-49 CrossRef
  12. Lynagh, T, Lynch, JW (2010) An improved ivermectin-activated chloride channel receptor for inhibiting electrical activity in defined neuronal populations. J Bio Chem 285: pp. 14890-14897 4/jbc.M110.107789" target="_blank" title="It opens in new window">CrossRef
  13. Tsien, JZ, Chen, DF, Gerber, D, Tom, C, Mercer, EH, Anderson, DJ, Mayford, M, Kandel, ER, Tonegawa, S (1996) Subregion- and cell type鈥搑estricted gene knockout in mouse brain. Cell 87: pp. 1317-1326 4(00)81826-7" target="_blank" title="It opens in new window">CrossRef
  14. Tang, W, Ehrlich, I, Wolff, SBE, Michalski, A-M, W枚lfl, S, Hasan, MT, L眉thi, A, Sprengel, R (2009) Faithful expression of multiple Proteins via 2A-peptide self-processing: a versatile and reliable method for manipulating brain circuits. J Neurosci 29: pp. 8621-8629 CrossRef
  15. Gorski, JA, Talley, T, Qiu, M, Puelles, L, Rubenstein, JLR, Jones, KR (2002) Cortical excitatory neurons and glia, but not GABAergic neurons, are produced in the Emx1-expressing lineage. J Neurosci 22: pp. 6309-6314
  16. Duty, S, Jenner, P (2011) Animal models of Parkinson鈥檚 disease: a source of novel treatments and clues to the cause of the disease. Brit J Pharmacol 164: pp. 1357-1391 476-5381.2011.01426.x" target="_blank" title="It opens in new window">CrossRef
  17. Hu, Z-L, Huang, Y, Tao, X-R, Qi, Z-H, Chen, J-Y, Ding, Y-Q (2012) Inducible Prrxl1-CreERT2 recombination activity in the somatosensory afferent pathway. Genesis 50: pp. 552-560 CrossRef
  18. Ding, Y-Q, Yin, J, Xu, H-M, Jacquin, MF, Chen, Z-F (2003) Formation of whisker-related principal sensory nucleus-based lemniscal pathway requires a paired homeodomain transcription factor, Drg11. J Neurosci 23: pp. 7246-7254
  19. Chen, Z-F, Rebelo, S, White, F, Malmberg, AB, Baba, H, Lima, D, Woolf, CJ, Basbaum, AI, Anderson, DJ (2001) The paired homeodomain protein DRG11 is required for the projection of cutaneous sensory afferent fibers to the dorsal spinal cord. Neuron 31: pp. 59-73 41-5" target="_blank" title="It opens in new window">CrossRef
  20. Monteirol, C, Rebelol, S, Galhardol, V, Reguengal, C, Limal, D (2011) Postnatal expression of the homeobox gene Prrxl1 (Drg11) is increased in inflammatory but not neuropathic pain. Eur J Pain 15: pp. 477-481 CrossRef
  21. Roder, JD, Stair, EL (1998) An overview of ivermectin toxicosis. Vet Hum Toxicol 40: pp. 369-370
  22. Davis, JA, Paylor, R, McDonald, MP, Libbey, M, Ligler, A, Bryant, K, Crawle, JN (1999) Behavioral effects of ivermectin in mice. Lab Anim Sci 49: pp. 288-296
  23. Yardley, MM, Wyatt, L, Khoja, S, Asatryan, L, Ramaker, MJ, Finn, DA, Alkana, RL, Huynh, N, Louie, SG, Petasis, NA, Bortolato, M, Davies, D (2012) Ivermectin reduces alcohol intake and preference in mice. Neuropharmacology 63: pp. 190-201 4" target="_blank" title="It opens in new window">CrossRef
  24. Adelsberger, H, Lepier, A, Dudel, J (2000) Activation of rat recombinant 伪1尾2纬2S GABAA receptor by the insecticide ivermectin. Eur J Pharmacol 394: pp. 163-170 4-2999(00)00164-3" target="_blank" title="It opens in new window">CrossRef
  25. Soriano, P (1999) Generalized lacZ expression with the ROSA26 Cre reporter strain. Nat Genet 21: pp. 70-71 CrossRef
  26. Auricchio, A, Hildinger, M, O鈥機onnor, E, Gao, GP, Wilson, JM (2001) Isolation of highly infectious and pure adeno-associated virus type 2 vectors with a single-step gravity-flow column. Hum Gene Ther 12: pp. 71-76 4303401450988" target="_blank" title="It opens in new window">CrossRef
  27. Grieger, JC, Choi, VW, Samulski, RJ (2006) Production and characterization of adeno-associated viral vectors. Nat Protoc 1: pp. 1412-1428 CrossRef
  28. Chaplan, SR, Bach, FW, Pogrel, JW, Chung, JM, Yaksh, TL (1994) Quantitative assessment of tactile allodynia in the rat paw. J Neurosci Meth 53: pp. 55-63 4)90144-9" target="_blank" title="It opens in new window">CrossRef
  29. Dai, JX, Han, HL, Tian, M, Cao, J, Xiu, JB, Song, NN, Huang, Y, Xu, TL, Ding, Y-Q, Xu, L (2008) Enhanced contextual fear memory in central serotonin-deficient mice. Proc Natl Acad Sci U S A 105: pp. 11981-11986 CrossRef
  
• 刊物主题：Neurosciences; Neurology;
• 出版者：BioMed Central
• ISSN：1756-6606

文摘

Background Genetic methods for inducibly and reversibly inhibiting neuronal activity of specific neurons are critical for exploring the functions of neuronal circuits. The engineered human glycine receptor, called ivermectin (IVM)-gated silencing receptor (IVMR), has been shown to possess this ability in vitro. Results Here we generated a mouse line, in which the IVMR coding sequence was inserted into the ROSA26 locus downstream of a loxP-flanked STOP cassette. Specific Cre-mediated IVMR expression was revealed by mis-expression of Cre in the striatum and by crossing with several Cre lines. Behavioral alteration was observed in Rosa26-IVMR mice with unilateral striatal Cre expression after systemic administration of IVM, and it could be re-initiated when IVM was applied again. A dramatic reduction in neuron firing was recorded in IVM-treated free moving Rosa26-IVMR;Emx1-Cre mice, and neuronal excitability was reduced within minutes as shown by recording in brain slice. Conclusion This Rosa26-IVMR mouse line provides a powerful tool for exploring selective circuit functions in freely behaving mice.