Alteration of immunoproteome profile of Echinococcus granulosus hydatid fluid with progression of cystic echinococcosis
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- 作者：Chun-Seob Ahn (1)
Xiumin Han (2)
Young-An Bae (3)
Xiao Ma (2)
Jin-Taek Kim (1)
Huixia Cai (1) (2)
Hyun-Jong Yang (4)
Insug Kang (5)
Hu Wang (2)
Yoon Kong (1)
1. Department of Molecular Parasitology ; Sungkyunkwan University School of Medicine and Center for Molecular Medicine ; Samsung Biomedical Research Institute ; Suwon ; 440-746 ; Korea
2. Qinghai Province Institute for Endemic Diseases Prevention and Control ; Xining ; Qinghai ; China
3. Department of Microbiology ; Graduate School of Medicine ; Gachon University ; Incheon ; Korea
4. Department of Parasitology ; Ewha Womans University ; School of Medicine ; Seoul ; Korea
5. Department of Molecular Biology and Biochemistry ; School of Medicine ; Kyung Hee University ; Seoul ; Korea - 关键词：Echinococcus granulosus ; Cystic echinococcosis ; Hydatid fluid ; Immunoproteome ; Antigen B ; Antigen 5 ; Stage ; specificity
- 刊名：Parasites & Vectors
- 出版年：2015
- 出版时间：December 2015
- 年：2015
- 卷：8
- 期：1
- 全文大小：2,710 KB
- 参考文献：1. Eckert J, Deplazes P: Biological, epidemiological, and clinical aspects of echinococcosis, a zoonosis of increasing concern. / Clin Microbiol Rev 2009, 27:107鈥?35.
2. Zhang W, Zhang Z, Wu W, Shi B, Li J, Zhou X, Wen H, McManus DP: Epidemiology and control of echinococcosis in central Asia, with particular reference to the People鈥檚 Republic of China. / Acta Trop 2014. http://dx.doi.org/10.1016/j.actatropica.2014.03.014.
3. Yang YR, Clements AC, Gray DJ, Atkinson JA, Williams GM, Barnes TS, McManus DP: Impact of anthropogenic and natural environmental changes on Echinococcus transmission in Ningxia Hui Autonomous Region, the People鈥檚 Republic of China. / Parasit Vectors 2012, 5:146. CrossRef
4. Craig PS, McManus DP, Lightowlers MW, Chabalgoity JA, Garcia HH, Gavidia CM, Gilman RH, Gonzalez AE, Lorca M, Naquira C, Nieto A, Schantz PM: Prevention and control of cystic echinococcosis. / Lancet Infect Dis 2007, 7:385鈥?94. CrossRef
5. Brunetti E, Kern P, Vuitton DA: Writing panel for the WHO-IWGE. expert consensus for the diagnosis and treatment of cystic and alveolar echinococcosis in humans. / Acta Trop 2010, 114:1鈥?6. CrossRef
6. Caremani M, Maestrini R, Occhini U, Sassoli S, Accorsi A, Giorgio A, Filice C: Echographic epidemiology of cystic hydatid disease in Italy. / Eur J Epidemiol 1993, 9:401鈥?04. CrossRef
7. Perdomo R, Alvarez C, Monti J, Ferreira C, Chiesa A, Carb贸 A, Alvez R, Grauert R, Stern D, Carmona C, Yarzabal L: Principles of the surgical approach in human liver cystic echinococcosis. / Acta Trop 1997, 64:109鈥?22. CrossRef
8. Eckert J, Gemmell MA, Meslin FX, Pawlowski ZS: / WHO/OIE Manual on Echinococcosis in Humans and Animals: a Public Health Problem of Global Concern. Paris: WHO/OIE; 2001.
9. Taheri MS, Pourghorban R, Faghihi Langroudi T, Shabestari AA, Haghighatkhah H, Bagheri SM: Not-to-tap cystic lesions: spectrum of hydatid disease. / Emerg Radiol 2013, 20:553鈥?61. CrossRef
10. Siracusano A, Margutti P, Delunardo F, Profumo E, Rigan貌 R, Buttari B, Teggi A, Ortona E: Molecular cross-talk in host-parasite relationships: the intriguing immunomodulatory role of Echinococcus antigen B in cystic echinococcosis. / Int J Parasitol 2008, 38:1371鈥?376. CrossRef
11. Ortona E, Rigan貌 R, Margutti P, Notargiacomo S, Ioppolo S, Vaccari S, Barca S, Buttari B, Profumo E, Teggi A, Siracusano A: Native and recombinant antigens in the immunodiagnosis of human cystic echinococcosis. / Parasite Immunol 2000, 22:553鈥?59. CrossRef
12. Lorenzo C, Last JA, Gonz谩lez-Sapienza GG: The immunogenicity of Echinococcus granulosus antigen 5 is determined by its post-translational modifications. / Parasitology 2005, 131:669鈥?77. CrossRef
13. Haag KL, Alves-Junior L, Zaha A, Ayala FJ: Contingent, non-neutral evolution in a multicellular parasite: natural selection and gene conversion in the Echinococcus granulosus antigen B gene family. / Gene 2004, 333:157鈥?67. CrossRef
14. Zhang W, Li J, Jones MK, Zhang Z, Zhao L, Blair D, McManus DP: The Echinococcus granulosus antigen B gene family comprises at least 10 unique genes in five subclasses which are differentially expressed. / PLoS Negl Trop Dis 2010, 4:e784. CrossRef
15. Shepherd JC, Aitken A, McManus DP: A protein secreted in vivo by Echinococcus granulosus inhibits elastase activity and neutrophil chemotaxis. / Mol Biochem Parasitol 1991, 44:81鈥?0. CrossRef
16. Daeki AO, Craig PS, Shambesh MK: IgG-subclass antibody responses and the natural history of hepatic cystic echinococcosis in asymptomatic patients. / Ann Trop Med Parasitol 2000, 94:319鈥?28.
17. Obal G, Ramos AL, Silva V, Lima A, Batthyany C, Bessio MI, Ferreira F, Salinas G, Ferreira AM: Characterisation of the native lipid moiety of Echinococcus granulosus antigen B. / PLoS Negl Trop Dis 2012, 6:e1642. CrossRef
18. Junghanss T, da Silva AM, Horton J, Chiodini PL, Brunetti E: Clinical management of cystic echinococcosis: state of the art, problems, and perspectives. / Am J Trop Med Hyg 2008, 79:301鈥?11.
19. Mariconti M, Bazzocchi C, Tamarozzi F, Meroni V, Genco F, Maserati R, Brunetti E: Immunoblotting with human native antigen shows stage-related sensitivity in the serodiagnosis of hepatic cystic echinococcosis. / Am J Trop Med Hyg 2014, 90:75鈥?9. CrossRef
20. Bae YA, Jeong YT, Chung JY, Kim SH, Mahanta J, Feng Z, Chong CK, Kim TS, Kong Y: A recombinant chimeric antigen toward a standardized serodiagnosis of Taenia solium neurocysticercosis. / Proteomics Clin Appl 2008, 2:1596鈥?610. CrossRef
21. Monteiro KM, Cardoso MB, Follmer C, da Silveira NP, Vargas DM, Kitajima EW, Zaha A, Ferreira HB: Echinococcus granulosus antigen B structure: subunit composition and oligomeric states. / PLoS Negl Trop Dis 2012, 6:e1551. CrossRef
22. Lightowlers MW, Liu DY, Haralambous A, Rickard MD: Subunit composition and specificity of the major cyst fluid antigens of Echinococcus granulosus . / Mol Biochem Parasitol 1989, 37:171鈥?82. CrossRef
23. Khabiri AR, Bagheri F, Assmar M, Siavashi MR: Analysis of specific IgE and IgG subclass antibodies for diagnosis of Echinococcus granulosus . / Parasite Immunol 2006, 28:357鈥?62. CrossRef
24. Ibrahem MM, Rafiei A, Dar FK, Azwai SM, Carter SD, Craig PS: Serodiagnosis of cystic echinococcosis in naturally infected camels. / Parasitology 2002, 125:245鈥?51. CrossRef
25. McManus DP: Immunodiagnosis of sheep infections with Echinococcus granulosus : in 35聽years where have we come? / Parasite Immunol 2004, 36:125鈥?30. CrossRef
26. Lee EG, Kim SH, Bae YA, Chung JY, Suh M, Na BK, Kim TS, Kang I, Ma L, Kong Y: A hydrophobic ligand binding protein of the Taenia solium metacestode mediates uptake of the host lipid: implication for the maintenance of parasitic cellular homeostasis. / Proteomics 2007, 7:4016鈥?030. CrossRef
27. Kim SH, Bae YA, Yang Y, Hong ST, Kong Y: Paralogous proteins comprising the 150聽kDa hydrophobic-ligand-binding-protein complex of the Taenia solium metacestode have evolved non-overlapped binding affinities toward fatty acid analogs. / Int J Parasitol 2011, 41:1207鈥?215. CrossRef
28. Virginio VG, Hern谩ndez A, Rott MB, Monteiro KM, Zandonai AF, Nieto A, Zaha A, Ferreira HB: A set of recombinant antigens from Echinococcus granulosus with potential for use in the immunodiagnosis of human cystic hydatid disease. / Clin Exp Immunol 2003, 132:309鈥?15. CrossRef
29. Virginio VG, Monteiro KM, Drumond F, de Carvalho MO, Vargas DM, Zaha A, Ferreira HB: Excretory/secretory products from in vitro-cultured Echinococcus granulosus protoscoleces. / Mol Biochem Parasitol 2012, 183:15鈥?2. CrossRef
30. Cui SJ, Xu LL, Zhang T, Xu M, Yao J, Fang CY, Feng Z, Yang PY, Hu W, Liu F: Proteomic characterization of larval and adult developmental stages in Echinococcus granulosus reveals novel insight into host-parasite interactions. / J Proteomics 2013, 84:158鈥?75. CrossRef
31. Gancedo C, Flores C: Moonlighting proteins in yeasts. / Microbiol Mol Biol Rev 2008, 72:197鈥?10. CrossRef
32. Pomel S, Luk FC, Beckers CJ: Host cell egress and invasion induce marked relocations of glycolytic enzymes in Toxoplasma gondii tachyzoites. / PLoS Pathog 2008, 4:e1000188. CrossRef
33. Lorenzatto KR, Monteiro KM, Paredes R, Paludo GP, da Fons锚ca MM, Galanti N, Zaha A, Ferreira HB: Fructose-bisphosphate aldolase and enolase from Echinococcus granulosus : genes, expression patterns and protein interactions of two potential moonlighting proteins. / Gene 2012, 506:76鈥?4. CrossRef
34. Buchner J, Grallert H, Jakob U: Analysis of chaperone function using citrate synthase as nonnative substrate protein. / Methods Enzymol 1998, 290:323鈥?38. CrossRef
35. Asahi H, Osman A, Cook RM, LoVerde PT, Stadecker MJ: Schistosoma mansoni phosphoenolpyruvate carboxykinase, a novel egg antigen: immunological properties of the recombinant protein and identification of a T-cell epitope. / Infect Immun 2000, 68:3385鈥?393. CrossRef
36. Monteiro KM, de Carvalho MO, Zaha A, Ferreira HB: Proteomic analysis of the Echinococcus granulosus metacestode during infection of its intermediate host. / Proteomics 2010, 10:1985鈥?999. CrossRef
37. Ambrosio J, Landa A, Merchant MT, Laclette JP: Protein uptake by cysticerci of Taenia crassiceps . / Arch Med Res 1994, 25:325鈥?30. - 刊物主题：Parasitology; Infectious Diseases; Tropical Medicine; Entomology;
- 出版者：BioMed Central
- ISSN：1756-3305
文摘
Background Cystic echinococcosis (CE), caused by Echinococcus granulosus metacestode, invokes a serious public health concern. Early diagnosis has great impacts on reduction of disability-adjusted life years. Several antigen B-related molecules (EgAgB; EgAgB1-5) are known to be immunopotent, but detection of EgAgB is variable in many patients and may not allow reliable interpretation of its immunological relevance. More importantly, the immunoproteome profile of hydatid fluid (HF) has not been addressed. Methods We conducted a proteome analysis of the HF of a single fertile cyst of CE1 and CE2 stages through two-dimensional electrophoresis (2-DE). Each protein spot was analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). We subsequently determined the immunoproteome profile employing patient sera of entire disease spectrum from CE1 to CE5 stages. Results We identified 40 parasite proteins, of which EgAgB (28 spots) and antigen 5 (EgAg5; 5 molecules) were abundant. EgAgB proteoforms constituted the majority, mostly EgAgB1 (24 spots), followed by EgAgB2 and EgAgB4 (2 spots each). EgAgB3 was detected only by liquid chromatography-MS/MS. EgAgB5 was not recognized. We also detected 38 host proteins, which were largely composed of serum components, antioxidant/xenobiotic enzymes, and enzymes involved in carbohydrate metabolism. CE1 and CE2 HF exhibited comparable spotting patterns, but CE2 HF harbored greater amounts of EgAgB and EgAg5 complexes. CE sera demonstrated complicated immune recognition patterns according to the disease progression; CE2 and CE3 stages exhibited strong antibody responses against diverse EgAgB and EgAg5 proteoforms, while CE1, CE4, and CE5 stages mainly reacted to EgAg5 and cathepsin B. Patient sera of alveolar echinococcosis (AE) cross-reacted with diverse EgAgB isoforms (36%). EgAg5 and cathepsin B also demonstrated cross-reactions with sera from neurocysticercosis and sparganosis. Conclusions Our results demonstrated that detection of a single defined molecule may not properly diagnose CE, since specific immunodominant epitopes changed as the disease progresses. Immunoproteome analysis combined with imaging studies may be practical in the differential diagnosis of CE from AE and other cystic lesions, as well as for staging CE, which are pertinent to establish appropriate patient management.