A reduction of viral mRNA, proteins and induction of altered morphogenesis reveals the anti-HTLV-1 activity of the labdane-diterpene myriadenolide in vitro

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• 作者：Camila Pacheco Silveira Martins (1) (2)
  Orlando Abreu Gomes (3)
  Marina Lobato Martins (2) (4)
  Luciana Debortoli de Carvalho (1) (2)
  Jaqueline Gontijo de Souza (1) (2)
  Flavio Guimaraes Da Fonseca (1)
  Rodrigo Gon莽alves Silva dos Santos (1) (2)
  Margareth Spangler Andrade (5)
  Carlos Leomar Zani (6)
  Elaine Maria de Souza-Fagundes (7)
  Edel Figueiredo Barbosa-Stancioli (1) (2)
  
  1. Laborat贸rio de Virologia B谩sica e Aplicada (LVBA) ; Departamento de Microbiologia ; Instituto de Ci锚ncias Biol贸gicas ; Universidade Federal de Minas Gerais ; Avenida Ant么nio Carlos ; 6627 ; Belo Horizonte ; Minas Gerais ; Brazil
  2. Interdisciplinary HTLV Research Group 鈥?GIPH - Funda莽茫o HEMOMINAS ; Belo Horizonte ; Minas Gerais ; Brazil
  3. N煤cleo de Ci锚ncias Exatas 鈥?FACE 鈥?Universidade FUMEC ; Belo Horizonte ; Minas Gerais ; Brazil
  4. Servi莽o de Pesquisa ; Funda莽茫o HEMOMINAS ; Belo Horizonte ; Minas Gerais ; Brazil
  5. Centro Tecnol贸gico SENAI CETEC ; Belo Horizonte ; Minas Gerais ; Brazil
  6. Centro de Pesquisas Ren猫 Rachou ; FIOCRUZ ; Belo Horizonte ; Minas Gerais ; Brazil
  7. Departamento de Fisiologia e Biof铆sica ; Instituto de Ci锚ncias Biol贸gicas ; Universidade Federal de Minas Gerais ; Belo Horizonte ; Minas Gerais ; Brazil
  
• 关键词：HTLV ; 1 ; Diterpene myriadenolide ; Antiviral activity ; HAM/TSP ; ATL
• 刊名：BMC Microbiology
• 出版年：2014
• 出版时间：December 2014
• 年：2014
• 卷：14
• 期：1
• 全文大小：2,370 KB
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• 刊物主题：Microbiology; Biological Microscopy; Fungus Genetics; Parasitology; Virology; Life Sciences, general;
• 出版者：BioMed Central
• ISSN：1471-2180

文摘

Background Human T-lymphotropic virus 1 (HTLV-1) has been associated with leukemia/lymphoma (ATL) and myelopathy/tropical spastic paraparesis (HAM/TSP), in addition to other inflammatory diseases as well as infection complications. Therapeutic approaches for HTLV-1-related pathologies are limited. The labdane diterpene myriadenolide (AMY) is a natural product that exhibit biological activities, such as anti-inflammatory and antiviral activity as reported for HIV and herpesvirus. Results We demonstrated that this natural product was able to inhibit the expression of gag-pol mRNA and substantially reduced the expression of the structural proteins p19 and gp46. Comparison of treated and untreated cells shows that AMY alters both the morphology and the release of viral particles. The Atomic Force Microscopy assay showed that the AMY treatment reduced the number of particles on the cell surface by 47%. Conclusion We demonstrated that the labdane diterpene myriadenolide reduced the expression of the structural proteins and the budding of viral particles, besides induces altered morphogenesis of HTLV-1, conferring on AMY a new antiviral activity that may be useful for the development of new compounds with specific anti-HTLV-1 activity.