Label-free proteomic analysis to confirm the predicted proteome of Corynebacterium pseudotuberculosis under nitrosative stress mediated by nitric oxide

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• 作者：Wanderson M Silva (59) (62) (63)
  Rodrigo D Carvalho (59)
  Siomar C Soares (59)
  Isabela FS Bastos (59)
  Edson L Folador (59)
  Gustavo HMF Souza (61)
  Yves Le Loir (62) (63)
  Anderson Miyoshi (59)
  Artur Silva (60)
  Vasco Azevedo (59)
  
  59. Depto de Biologia Geral ; Instituto de Ci锚ncias Biol贸gicas ; Universidade Federal de Minas Gerais ; Belo Horizonte ; Brazil
  62. Institut National de la Recherche Agronomique - INRA ; UMR1253 STLO ; Rennes ; 35042 ; France
  63. Agrocampus Ouest ; UMR1253 STLO ; Rennes ; 35042 ; France
  61. Waters Corporation ; MS Applications and Development Laboratory ; S茫o Paulo ; Brazil
  60. Instituto de Ci锚ncias Biol贸gicas ; Universidade Federal do Par谩 ; Bel茅m ; Par谩 ; Brazil
  
• 关键词：Corynebacterium pseudotuberculosis ; Caseous lymphadenitis ; Proteomics ; Label ; free proteomics ; Nitrosative stress ; Nitric oxide
• 刊名：BMC Genomics
• 出版年：2014
• 出版时间：December 2014
• 年：2014
• 卷：15
• 期：1
• 全文大小：3,094 KB
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• 刊物主题：Life Sciences, general; Microarrays; Proteomics; Animal Genetics and Genomics; Microbial Genetics and Genomics; Plant Genetics & Genomics;
• 出版者：BioMed Central
• ISSN：1471-2164

文摘

Background Corynebacterium pseudotuberculosis biovar ovis is a facultative intracellular pathogen, and the etiological agent of caseous lymphadenitis in small ruminants. During the infection process, the bacterium is subjected to several stress conditions, including nitrosative stress, which is caused by nitric oxide (NO). In silico analysis of the genome of C. pseudotuberculosis ovis 1002 predicted several genes that could influence the resistance of this pathogen to nitrosative stress. Here, we applied high-throughput proteomics using high definition mass spectrometry to characterize the functional genome of C. pseudotuberculosis ovis 1002 in the presence of NO-donor Diethylenetriamine/nitric oxide adduct (DETA/NO), with the aim of identifying proteins involved in nitrosative stress resistance. Results We characterized 835 proteins, representing approximately 41% of the predicted proteome of C. pseudotuberculosis ovis 1002, following exposure to nitrosative stress. In total, 102 proteins were exclusive to the proteome of DETA/NO-induced cells, and a further 58 proteins were differentially regulated between the DETA/NO and control conditions. An interactomic analysis of the differential proteome of C. pseudotuberculosis in response to nitrosative stress was also performed. Our proteomic data set suggested the activation of both a general stress response and a specific nitrosative stress response, as well as changes in proteins involved in cellular metabolism, detoxification, transcriptional regulation, and DNA synthesis and repair. Conclusions Our proteomic analysis validated previously-determined in silico data for C. pseudotuberculosis ovis 1002. In addition, proteomic screening performed in the presence of NO enabled the identification of a set of factors that can influence the resistance and survival of C. pseudotuberculosis during exposure to nitrosative stress.