Recent advances in in vivo applications of intein-mediated protein splicing
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- 作者：Natalya I Topilina (3)
Kenneth V Mills (4) - 关键词：Intein ; Protein splicing ; Post ; translational modification ; Protein purification ; Trans ; splicing ; Conditional protein splicing ; Trans ; genes ; Biosensor
- 刊名：Mobile DNA
- 出版年：2014
- 出版时间：December 2014
- 年：2014
- 卷：5
- 期：1
- 全文大小：1,001 KB
- 作者单位：Natalya I Topilina (3)
Kenneth V Mills (4)
3. Department of Biological Sciences, University at Albany, State University of New York, 1400 Washington Avenue, Albany, NY, 12222, USA
4. Department of Chemistry, College of the Holy Cross, 1 College Street, Worcester, MA, 01610, USA - ISSN：1759-8753
文摘
Intein-mediated protein splicing has become an essential tool in modern biotechnology. Fundamental progress in the structure and catalytic strategies of cis- and trans-splicing inteins has led to the development of modified inteins that promote efficient protein purification, ligation, modification and cyclization. Recent work has extended these in vitro applications to the cell or to whole organisms. We review recent advances in intein-mediated protein expression and modification, post-translational processing and labeling, protein regulation by conditional protein splicing, biosensors, and expression of trans-genes.