In vitro control of parasitic nematodes of small ruminants using some plant species containing flavonoids

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• 作者：Sylvester W. Fomum ; Ignatius V. Nsahlai
• 关键词：Control ; Crude extract ; Flavonoids ; Nematodes ; Plant species and Small ruminants
• 刊名：Tropical Animal Health and Production
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：49
• 期：2
• 页码：375-382
• 全文大小：
• 刊物类别：Medicine
• 刊物主题：Zoology; Veterinary Medicine/Veterinary Science;
• 出版者：Springer Netherlands
• ISSN：1573-7438
• 卷排序：49

文摘

This study determined in vitro anthelmintic efficacy of three plant species: Trema orientalis, Urtica dioica and Zanthozylum capense on nematode larvae of small ruminants. Dried leaf samples (40 g) were extracted in 70% ethanol, in portions of 10 g and concentrated to 100 ml. Half and one quarter of the original crude extract were both made to 100 ml. Rectal faecal material from 10 Merino sheep and 25 Nguni goats was pooled within species and thoroughly hand-mixed. Dung samples, each of 5 g were cultured for 12 days at 27 °C. On day 13, 4 plates were watered and 4 others treated with ethanol to correct for solvent effect on mortality. The design was 2 (animal species) × 3 (plant species) × 3 (extract concentrations). In each of three runs, three plates were treated with each crude extract in three incremental concentrations. Surviving L3 larvae were isolated, counted and mortalities became indices of anthelmintic efficacy. Data from nematode larval mortality were analysed to determine the effect of animal species, plant species, concentration and their interactions. Efficacy was affected by concentration (P = 0.0001), animal species (P = 0.0046), plant species (P = 0.0572), the interactions of animal species and concentration (P = 0.0010), plant species and concentration (P = 0.0123) and concentration × animal × plant species (P = 0.0435).