Amplicon-Based RNA Interference Targeting V2 Gene of Cotton Leaf Curl Kokhran Virus-Burewala Strain Can Provide Resistance in Transgenic Cotton Plants

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• 作者：Aneela Yasmeen ; Sarfraz Kiani ; Afshan Butt ; Abdul Qayyum Rao…
• 关键词：Gene transformation ; siRNA ; CLCuD ; Begomovirus ; V2 ORF ; Knockdown
• 刊名：Molecular Biotechnology
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：58
• 期：12
• 页码：807-820
• 全文大小：2,614 KB
• 刊物主题：Biotechnology; Biochemistry, general; Cell Biology; Protein Science; Biological Techniques; Human Genetics;
• 出版者：Springer US
• ISSN：1559-0305
• 卷排序：58

文摘

The conserved coat or V2 gene of begomoviruses is responsible for viral movement in the plant cells. RNAi technology was used to silence V2 gene for resistance against these viruses in transgenic plants. The transformation of the RNAi-based gene construct targeting V2 gene of CLCuKoV-Bur, cloned under 35S promoter, was done in two elite cotton varieties MNH-786 and VH-289 using shoot apex cut method of gene transformation. The transformation efficiency was found to be 3.75 and 2.88 % in MNH-786 and VH-289, respectively. Confirmation of successful transformation was done through PCR in T₀, T₁, and T₂ generations using gene-specific primers. Transgenic cotton plants were categorized on the basis of the virus disease index in T₁ generation. Copy number and transgene location were observed using FISH and karyotyping in T₂ generation which confirmed random integration of V2 RNAi amplicon at chromosome 6 and 16. Real-time quantitative PCR analyses of promising transgenic lines showed low virus titer compared to wild-type control plants upon challenging them with viruliferous whiteflies in a contained environment. From the results, it was concluded that amplicon V2 RNAi construct was able to limit virus replication and can be used to control CLCuV in the field.