Discriminating power of microsatellites in cranberry organelles for taxonomic studies in Vaccinium and Ericaceae

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• 作者：Brandon Schlautman ; Giovanny Covarrubias-Pazaran…
• 关键词：Cross ; species amplification ; Ericaceae ; Mitochondria ; Organelle markers ; Plastid ; Phylogenetic analysis ; SSR markers ; Vaccinium
• 刊名：Genetic Resources and Crop Evolution
• 出版年：2017
• 出版时间：March 2017
• 年：2017
• 卷：64
• 期：3
• 页码：451-466
• 全文大小：
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Plant Sciences; Plant Systematics/Taxonomy/Biogeography; Plant Genetics & Genomics; Plant Physiology; Agriculture;
• 出版者：Springer Netherlands
• ISSN：1573-5109
• 卷排序：64

文摘

Simple sequence repeats (SSRs) in chloroplast and mitochondrial DNA, which have not been previously developed in the Ericaceae or, more specifically, in the genus Vaccinium, can be powerful tools for determining evolutionary relationships among taxa. In this study, 30 chloroplast, 23 mitochondrial, and 1 mitochondrion-like SSRs were identified in cranberry (V. macrocarpon), and primer-pairs were developed and tested for each locus. Although no polymorphisms were detected for any of the 54 SSR loci in nine diverse cranberry genotypes, all primers were cross-transferable to some extent to a panel of 12 additional Vaccinium taxa and four non-Vaccinium Ericaceae species. A Neighbor-Joining tree of the estimated average squared distances resolved the species by genus and by section within Vaccinium. Similar topologies with increased branch support were observed in Bayesian inference trees constructed from the DNA sequences of six plastid and two mitochondrial SSR loci. Two multiplexing/poolplexing panels of M13 fluorescently labeled primers, which amplify 24 of the 54 markers, were developed and can serve as an efficient, cost-effective means for characterizing the basic molecular phylogeny of Vaccinium. Increased understanding of evolutionary relationships among Vaccinium species should facilitate interspecific hybridization and introgression efforts to improve economically important traits of commercial berry crops.