A fast and effective determination of the biodistribution and subcellular localization of fluorescent immunoliposomes in freshly excised animal organs
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- 作者：Felista L. Tansi ; Ronny Rüger ; Ansgar M. Kollmeier ; Claudia Böhm…
- 关键词：Biodistribution studies ; Fluorescence and autofluorescence imaging ; Molecular targeting ; Liposomes
- 刊名：BMC Biotechnology
- 出版年：2017
- 出版时间：December 2017
- 年：2017
- 卷：17
- 期：1
- 全文大小：3596KB
- 刊物类别：Chemistry and Materials Science
- 刊物主题：Applied Microbiology; Biotechnology; Biochemical Engineering; Genetic Engineering; Plant Breeding/Biotechnology; Biomedical Engineering/Biotechnology;
- 出版者：BioMed Central
- ISSN：1472-6750
- 卷排序：17
文摘
BackgroundPreclinical research implementing fluorescence-based approaches is inevitable for drug discovery and technology. For example, a variety of contrast agents developed for biomedical imaging are usually evaluated in cell systems and animal models based on their conjugation to fluorescent dyes. Biodistribution studies of excised organs are often performed by macroscopic imaging, whereas the subcellular localization though vital, is often neglected or further validated by histological procedures. Available systems used to define the subcellular biodistribution of contrast agents such as intravital microscopes or ex vivo histological analysis are expensive and not affordable by the majority of researchers, or encompass tedious and time consuming steps that may modify the contrast agents and falsify the results. Thus, affordable and more reliable approaches to study the biodistribution of contrast agents are required. We developed fluorescent immunoliposomes specific for human fibroblast activation protein and murine endoglin, and used macroscopic fluorescence imaging and confocal microscopy to determine their biodistribution and subcellular localization in freshly excised mice organs at different time points post intravenous injection.