Effect of chromate ions on marine microalgae Phaeodactylum tricornutum

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• 作者：D. T. Gabbasova ; D. N. Matorin ; I. V. Konyukhov ; N. Kh. Seifullina…
• 关键词：Phaeodactylum tricornutum ; chromate ions ; chlorophyll fluorescence ; photosynthesis ; biotesting ; JIP test ; M ; PEA ; 2
• 刊名：Microbiology
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：86
• 期：1
• 页码：64-72
• 全文大小：
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Microbiology; Medical Microbiology;
• 出版者：Pleiades Publishing
• ISSN：1608-3237
• 卷排序：86

文摘

Effect of chromate ions on the culture of a marine diatom Phaeodactylum tricornutum was studied using an M-PEA-2 fluorimeter, which carries out simultaneous measurement of fluorescence induction and redox transformations of the P₇₀₀ pigment within a millisecond range. Chromate ions were shown to inhibit electron transport in PS II and decrease the rate of Q_А reduction. This results in decreased values of the quantum yield of electron transport in PS II (ϕ_Eo) and performance index (PI_ABS), lower rates of P700 reduction, and increased energy (DI0/RC) and ΔpH-dependent nonphotochemical quenching (q_E). Emergence of the slow component of P₇₀₀ reduction was observed, indicating the activation of cyclic transport in the presence of chromate. Performance index (PI_ABS), which was the most sensitive parameter, may be recommended for detection of chromate ions at early stages of their toxic action. The fluorescence parameter F_O is promising application in biotesting to assess the algal growth rates.