DNA barcoding of fishes in Irtysh River China

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• 作者：Tianyan Yang ; Wei Meng ; Renming Zhang ; Tianxiang Gao…
• 关键词：Irtysh River ; fishes DNA barcoding ; species identification ; cryptic species
• 刊名：Russian Journal of Genetics
• 出版年：2016
• 出版时间：September 2016
• 年：2016
• 卷：52
• 期：9
• 页码：969-976
• 全文大小：226 KB
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine
  Human Genetics
  Animal Genetics and Genomics
  Microbial Genetics and Genomics
  Russian Library of Science
  
• 出版者：MAIK Nauka/Interperiodica distributed exclusively by Springer Science+Business Media LLC.
• ISSN：1608-3369
• 卷排序：52

文摘

DNA barcoding was a molecular diagnostic method that provided rapid and accurate species identification. The 650 bp-length cytochrome c oxidase subunit I (COI) gene of 33 species in Irtysh River China was sequenced and analyzed in this study. The average intra-species, -genus, -family, and -order of Kimura two parameter (K2P) distances were 0.003, 0.060, 0.163 and 0.240, respectively. The genetic distance between genus Barbatula and Cobitis was the largest whereas that between genus Hypophthalmichthys and Aristichthys was the smallest. The neighbour-joining tree constructed by all 44 haplotypes was divided into two major clusters: Cypriniformes fishes and other fishes. A cryptic species of Barbatula barbatula was detected according to 2% genetic threshold.