Evaluation of behavior and expression of NaV1.7 in dorsal root ganglia after sciatic nerve compression and application of nucleus pulposus in rats

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• 作者：Michiaki Mukai (1)
  Yoshihiro Sakuma (1)
  Miyako Suzuki (1)
  Sumihisa Orita (1)
  Kazuyo Yamauchi (1)
  Gen Inoue (1)
  Yasuchika Aoki (1)
  Tetsuhiro Ishikawa (1)
  Masayuki Miyagi (1)
  Hiroto Kamoda (1)
  Gou Kubota (1)
  Yasuhiro Oikawa (1)
  Kazuhide Inage (1)
  Takeshi Sainoh (1)
  Jun Sato (1)
  Junichi Nakamura (1)
  Masashi Takaso (1)
  Tomoaki Toyone (1)
  Kazuhisa Takahashi (1)
  Seiji Ohtori (1)
  
• 关键词：Rat ; Pain ; Nerve ; Compression ; NaV1.7 ; Nucleus pulposus
• 刊名：European Spine Journal
• 出版年：2014
• 出版时间：February 2014
• 年：2014
• 卷：23
• 期：2
• 页码：463-468
• 全文大小：276 KB
• 作者单位：Michiaki Mukai (1)
  Yoshihiro Sakuma (1)
  Miyako Suzuki (1)
  Sumihisa Orita (1)
  Kazuyo Yamauchi (1)
  Gen Inoue (1)
  Yasuchika Aoki (1)
  Tetsuhiro Ishikawa (1)
  Masayuki Miyagi (1)
  Hiroto Kamoda (1)
  Gou Kubota (1)
  Yasuhiro Oikawa (1)
  Kazuhide Inage (1)
  Takeshi Sainoh (1)
  Jun Sato (1)
  Junichi Nakamura (1)
  Masashi Takaso (1)
  Tomoaki Toyone (1)
  Kazuhisa Takahashi (1)
  Seiji Ohtori (1)
  
  1. Department of Orthopaedic Surgery, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba, 260-8670, Japan
  
• ISSN：1432-0932

文摘

Purpose The pathomechanisms of pain resulting from lumbar disc herniation have not been fully elucidated. Prostaglandins and cytokines generated at the inflammatory site produce associated pain; however, non-steroidal anti-inflammatory drugs and steroids are sometimes ineffective in patients. Tetrodotoxin-sensitive voltage-gated sodium (NaV) channels are related to sensory transmission in primary sensory nerves. The sodium channel NaV1.7 has emerged as an attractive analgesic target. The purpose of this study was to evaluate pain-related behavior and expression of NaV1.7 in dorsal root ganglia (DRG) after combined sciatic nerve compression and nucleus pulposus (NP) application in rats. Methods Rats were divided into three groups and underwent either sciatic nerve compression with NP for 2?s using forceps (n?=?20), sham operation with neither compression nor NP (n?=?20), or no operation (controls, n?=?20). Mechanical hyperalgesia was measured every second day for three weeks using von Frey filaments. NaV1.7 expression in L5 DRG was examined 7 and 14?days after surgery using immunohistochemistry. The number of neurons immunoreactive for NaV1.7 was compared among the three groups. Results Mechanical hyperalgesia was found over the 14-day observation in the nerve compression plus NP application group, but not in the sham-operated or control groups (P?<?0.05). NaV1.7 expression in L5 DRG was up-regulated in the nerve compression plus NP application group, compared with sham-operated and control rats (P?<?0.01). Conclusions Our results indicate that nerve compression plus NP application produces pain-related behavior. We conclude that NaV1.7 expression in DRG neurons may play an important role in mediating pain from sciatic nerves after compression injury and exposure to NP.