Xylanase (GH11) from Acremonium cellulolyticus: homologous expression and characterization

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• 作者：Masahiro Watanabe (1)
  Hiroyuki Inoue (1)
  Benchaporn Inoue (1)
  Miho Yoshimi (1)
  Tatsuya Fujii (1)
  Kazuhiko Ishikawa (1)
  
• 关键词：Acremonium cellulolyticus ; Xylanase ; Hemicellulose ; Homologous expression ; Biomass ; Xylan
• 刊名：AMB Express
• 出版年：2014
• 出版时间：December 2014
• 年：2014
• 卷：4
• 期：1
• 全文大小：1,025 KB
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• 作者单位：Masahiro Watanabe (1)
  Hiroyuki Inoue (1)
  Benchaporn Inoue (1)
  Miho Yoshimi (1)
  Tatsuya Fujii (1)
  Kazuhiko Ishikawa (1)
  
  1. Biomass Refinery Research Center, National Institute of Advanced Industrial Science and Technology (AIST), 3-11-32 Kagamiyama, Higashi-Hiroshima, Hiroshima, 739-0046, Japan
  
• ISSN：2191-0855

文摘

Cellulosic materials constitute most of the biomass on earth, and can be converted into biofuel or bio-based materials if fermentable sugars can be released using cellulose-related enzymes. Acremonium cellulolyticus is a mesophilic fungus which produces a high amount of cellulose-related enzymes. In the genome sequence data of A. cellulolyticus, ORFs showing homology to GH10 and GH11 xylanases were found. The xylanases of A. cellulolyticus play an important role in cellulolytic biomass degradation. Search of a draft genome sequence of A. cellulolyticus for xylanase coding regions identified seven ORFs showing homology to GH 11 xylanase genes (xylA, xylB, xylC, xylD, xylE, xylF and xylG). These genes were cloned and their enzymes were prepared with a homologous expression system under the control of a glucoamylase promoter. Six of the seven recombinant enzymes were successfully expressed, prepared, and characterized. These enzymes exhibited optimal xylanase activity at pH?4.0 -4.5. But this time, we found that only XylC had enormously higher relative activity (2947 U?mg ?) than the other xylanases at optimum pH. This result is surprising because XylC does not retain a carbohydrate-binding module 1 (CBM-1) that is necessary to bind tightly own substrate such as xylan. In this study, we discuss the relationship between activity, pH and sequence of seven xylanases in A. cellulolyticus.