Dietary soy isoflavones increase metastasis to lungs in an experimental model of breast cancer with bone micro-tumors

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• 作者：Xujuan Yang ; Aashvini Belosay ; James A. Hartman…
• 关键词：Dietary soy isoflavones ; Breast cancer ; Bone micro ; metastasis ; Lung metastasis
• 刊名：Clinical & Experimental Metastasis
• 出版年：2015
• 出版时间：April 2015
• 年：2015
• 卷：32
• 期：4
• 页码：323-333
• 全文大小：1,192 KB
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• 刊物主题：Cancer Research; Biomedicine general; Oncology; Hematology; Surgical Oncology;
• 出版者：Springer Netherlands
• ISSN：1573-7276

文摘

Bone is one of the most common sites for metastasis in breast cancer (BC). Micro-metastasis in bone marrow was detected in 30?% of patients with stage I, II, or III BC at primary surgery and is a strong indicator of poor prognosis. The role dietary soy isoflavones play in BC with bone micro-metastasis is unclear. In this study, we examined the effects of genistein, daidzein, (?-equol or a mixture of soy isoflavones on BC with bone micro-metastasis using an experimental model of murine mammary cancer 4T1 cells engineered with luciferase. A small number (1000) of 4T1 cells were injected into the tibia of female Balb/c mice to establish micro-tumors in bone. Soy isoflavones were supplemented in the AIN-93G diet at 750?mg/kg and were provided to mice from 3?weeks before to 3?weeks after cell injection. Bioluminescent imaging was conducted on day 2 (D2), D6, D8, D16 and D20 post cell injection and the results indicated dietary soy isoflavones enhanced the growth of bone micro-tumors on D8. Furthermore, dietary soy isoflavones stimulated metastatic tumor formation in lungs and increased Ki-67 protein expression in these metastasized tumors. In vitro, soy isoflavones (