Establishment and characterization of a clear cell carcinoma cell line, designated NOCC, derived from human ovary

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• 作者：Akihiro Ohyama ; Junko Toyomura ; Toshiaki Tachibana ; Seiji Isonishi…
• 关键词：Ovarian clear cell carcinoma ; NOCC cell line ; Transplantation ; VEGF ; HIF ; 1α
• 刊名：Human Cell
• 出版年：2016
• 出版时间：October 2016
• 年：2016
• 卷：29
• 期：4
• 页码：188-196
• 全文大小：2,164 KB
• 刊物主题：Cell Biology; Embryology; Oncology; Stem Cells; Reproductive Medicine; Cell Culture;
• 出版者：Springer Japan
• ISSN：1749-0774
• 卷排序：29

文摘

A cell line, designated NOCC, was established from the ascites of a patient with clear cell adenocarcinoma of the ovary. The cell line has been grown without interruption and continuously propagated by serial passaging (more than 76 times) over 7 years. The cells are spherical to polygonal-shaped, display neoplastic, and pleomorphic features, and grow in a jigsaw puzzle-like pattern while forming monolayers without contact inhibition. The cells proliferate rapidly, but are easily floated as a cell sheet. The population doubling time is about 29 h. The number of chromosomes ranges from 60 to 83. The modal number of chromosomes is 70–74 at the 30th passage. NOCC cells secreted 750.5 ng/ml of VEGF over 3 days of culture. Hypoxia inducible factor-1α (HIF-1α) is a primary regulator of VEGF under hypoxic conditions. NOCC cells were not sensitive to the anticancer drugs BEV, DOX, GEM, ETP, CDDP, or TXT. The graft of NOCC cells to a scid mouse displayed similar histological aspects to the original tumor. Both the NOCC cells and the graft of the NOCC cells gave a positive PAS reaction.