Effects of isoprenaline on endothelial connexins and angiogenesis in a human endothelial cell culture system
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- 作者：Stefan Dhein ; Christiane Gaertner…
- 关键词：Angiogenesis ; Gap junction ; Isoprenaline ; Beta ; adrenoceptor ; Connexin ; Intercellular communication
- 刊名：Naunyn-Schmiedeberg's Archives of Pharmacology
- 出版年：2015
- 出版时间：January 2015
- 年：2015
- 卷：388
- 期：1
- 页码：101-108
- 全文大小：1,027 KB
- 参考文献：1. Burnier L, Fontana P, Angelillo-Scherrer A, Kwak BR (2009) Intercellular communication in atherosclerosis. Physiology (Bethesda) 24:36-4 CrossRef
2. Ciccarelli M, Sorriento D, Cipolletta E, Santulli G, Fusco A, Zhou RH, Eckhart AD, Peppel K, Koch WJ, Trimarco B, Iaccarino G (2011) Impaired neoangiogenesis in β2-adrenoceptor gene-deficient mice: restoration by intravascular human β2-adrenoceptor gene transfer and role of NFκB and CREB transcription factors. Br J Pharmacol 162(3):712-21 CrossRef
3. de Wit C, Roos F, Bolz SS, Kirchhoff S, Kruger O, Willecke K, Pohl U (2000) Impaired conduction of vasodilation along arterioles in connexin40-deficient mice. Circ Res 86:649-55 CrossRef
4. de Wit C, Boettcher M, Schmidt VJ (2008) Signaling across myoendothelial gap junctions–fact or fiction? Cell Commun Adhes 15(3):231-45 CrossRef
5. Duerrschmidt N, Hagen A, Gaertner C, Wermke A, Nowicki M, Spanel-Borowski K, Stepan H, Mohr FW, Dhein S (2012) Nicotine effects on human endothelial intercellular communication via α4β2 and α3β2 nicotinic acetylcholine receptor subtypes. Naunyn Schmiedeberg’s Arch Pharmacol 385(6):621-32 CrossRef
6. Ferro A, Queen LR, Priest RM, Xu B, Ritter JM, Poston L, Ward JP (1999) Activation of nitric oxide synthase by beta 2-adrenoceptors in human umbilical vein endothelium in vitro. Br J Pharmacol 126(8):1872-880 CrossRef
7. G?rtner C, Ziegelh?ffer B, Kostelka M, Stepan H, Mohr FW, Dhein S (2012) Knock-down of endothelial connexins impairs angiogenesis. Pharmacol Res 65(3):347-57 CrossRef
8. Haussig S, Schubert A, Mohr FW, Dhein S (2008) Sub-chronic nicotine exposure induces intercellular communication failure and differential down-regulation of connexins in cultured human endothelial cells. Atherosclerosis 196(1):210-18 CrossRef
9. Hervé JC, Dhein S (2010) Peptides targeting gap junctional structures. Curr Pharm Des 16(28):3056-070 CrossRef
10. Ishii M, Mueller I, Nakajima T, Pasquale EB, Ogawa K (2011) EphB signaling inhibits gap junctional intercellular communication and synchronized contraction in cultured cardiomyocytes. Basic Res Cardiol 106(6):1057-068 CrossRef
11. Lamy S, Lachambre MP, Lord-Dufour S, Béliveau R (2010) Propranolol suppresses angiogenesis in vitro: inhibition of proliferation, migration, and differentiation of endothelial cells. Vasc Pharmacol 53(5-):200-08 CrossRef
12. Langlois S, Cowan KN, Shao Q, Cowan BJ, Laird DW (2008) Caveolin-1 and -2 interact with connexin43 and regulate gap junctional intercellular communication in keratinocytes. Mol Biol Cell 19(3):912-28 CrossRef
13. Laranjo S, Costa G, Paramés F, Freitas I, Martins JD, Trigo C, Pinto FF (2014) The role of propranolol in the treatment of infantile hemangioma. Rev Port Cardiol 33(5):289-95
14. Liao Y, Day KH, Damon DN, Duling BR (2001) Endothelial cell-specific knockout of connexin 43 causes hypotension and bradycardia in mice. Proc Natl Acad Sci U S A 98:9989-994 CrossRef
15. Looft-Wilson RC, Billaud M, Johnstone SR, Straub AC, Isakson BE (2012) Interaction between nitric oxide signaling and gap junctions: effects on vascular function. Biochim Biophys Acta 1818(8):1895-902 Cr - 作者单位：Stefan Dhein (1)
Christiane Gaertner (1)
Christian Georgieff (1)
Aida Salameh (2)
Franziska Schlegel (1) (3)
Friedrich-Wilhelm Mohr (1)
1. Clinic for Cardiac Surgery, Heart Center Leipzig, University of Leipzig, Struempellstr. 39, 04289, Leipzig, Germany
2. Clinic for Pediatric Cardiology, University of Leipzig Heart Center, Leipzig, Germany
3. Translational Centre for Regenerative Medicine TRM, University Leipzig, Leipzig, Germany - 刊物类别：Biomedical and Life Sciences
- 刊物主题：Biomedicine
Pharmacology and Toxicology
Neurosciences - 出版者：Springer Berlin / Heidelberg
- ISSN：1432-1912
文摘
Downregulation of endothelial connexins has been shown to result in impaired angiogenesis. Isoprenaline is known to upregulate Cx43 in cardiomyocytes. Effects of isoprenaline on endothelial connexins are unknown. We wanted to investigate whether isoprenaline might induce upregulation of connexins Cx37, Cx40, or Cx43 in human endothelial cells and whether it may promote angiogenesis. Human umbilical vein endothelial cells (HUVECs) were cultured until confluence (5?days) and subsequently seeded in Matrigel in vitro angiogenesis assays for 18?h. During the entire cell culture and angiogenesis period, cells were treated with vehicle or isoprenaline (100?nM). Finally, the resulting angiogenetic network was investigated (immuno)histologically. Moreover, expression of Cx37, Cx40, and Cx43 was determined by Western blot. In addition, we measured functional intercellular gap junction coupling by dye injection using patch clamp technique. Isoprenaline resulted in significantly enhanced expression of endothelial Cx43 and to a lower degree of Cx40 and Cx37. The number of coupling cells was significantly increased. Regarding angiogenesis, we observed significantly enhanced formation of branches and a higher complexity of the tube networks with more branches/length. Isoprenaline increases endothelial connexin expression and intercellular coupling and promotes tube formation.