Pulsed 13C2-Acetate Protein-SIP Unveils Epsilonproteobacteria as Dominant Acetate Utilizers in a Sulfate-Reducing Microbial Community Mineralizing Benzene
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- 作者：Robert Starke ; Andreas Keller ; Nico Jehmlich ; Carsten Vogt…
- 关键词：Anaerobic benzene degradation ; Metaproteomics ; Carbon flow
- 刊名：Microbial Ecology
- 出版年：2016
- 出版时间：May 2016
- 年：2016
- 卷：71
- 期：4
- 页码：901-911
- 全文大小：700 KB
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Andreas Keller (3) (4)
Nico Jehmlich (2)
Carsten Vogt (3)
Hans H. Richnow (3)
Sabine Kleinsteuber (4)
Martin von Bergen (2) (5)
Jana Seifert (1)
1. Institute for Animal Science, University of Hohenheim, Emil-Wolff-Str. 6-10, 70599, Stuttgart, Germany
2. Department of Proteomics, Helmholtz-Centre for Environmental Research (UFZ), Permoserstr. 15, 04318, Leipzig, Germany
3. Department of Isotope Biogeochemistry, Helmholtz-Centre for Environmental Research (UFZ), Permoserstr. 15, 04318, Leipzig, Germany
4. Department of Environmental Microbiology, Helmholtz-Centre for Environmental Research (UFZ), Permoserstr. 15, 04318, Leipzig, Germany
5. Department of Metabolomics, Helmholtz-Centre for Environmental Research (UFZ), Permoserstr. 15, 04318, Leipzig, Germany - 刊物类别：Biomedical and Life Sciences
- 刊物主题：Life Sciences
Microbiology
Ecology
Geoecology and Natural Processes
Nature Conservation - 出版者：Springer New York
- ISSN：1432-184X
文摘
In a benzene-degrading and sulfate-reducing syntrophic consortium, a clostridium affiliated to the genus Pelotomaculum was previously described to ferment benzene while various sulfate-reducing Deltaproteobacteria and a member of the Epsilonproteobacteria were supposed to utilize acetate and hydrogen as key metabolites derived from benzene fermentation. However, the acetate utilization network within this community was not yet unveiled. In this study, we performed a pulsed 13C2-acetate protein stable isotope probing (protein-SIP) approach continuously spiking low amounts of acetate (10 μM per day) in addition to the ongoing mineralization of unlabeled benzene. Metaproteomics revealed high abundances of Clostridiales followed by Syntrophobacterales, Desulfobacterales, Desulfuromonadales, Desulfovibrionales, Archaeoglobales, and Campylobacterales. Pulsed acetate protein-SIP results indicated that members of the Campylobacterales, the Syntrophobacterales, the Archaeoglobales, the Clostridiales, and the Desulfobacterales were linked to acetate utilization in descending abundance. The Campylobacterales revealed the fastest and highest 13C incorporation. Previous experiments suggested that the activity of the Campylobacterales was not essential for anaerobic benzene degradation in the investigated community. However, these organisms were consistently detected in various hydrocarbon-degrading and sulfate-reducing consortia enriched from the same aquifer. Here, we demonstrate that this member of the Campylobacterales is the dominant acetate utilizer in the benzene-degrading microbial consortium.