Age-related differences in human palatine tonsillar B cell subsets and immunoglobulin isotypes

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• 作者：Jino Lee ; Dong-Yeop Chang ; Sang-Wook Kim…
• 关键词：Age ; Tonsil ; B cell ; Subset ; Immunoglobulin ; Isotype
• 刊名：Clinical and Experimental Medicine
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：16
• 期：1
• 页码：81-87
• 全文大小：1,308 KB
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• 作者单位：Jino Lee (1)
  Dong-Yeop Chang (1) (2)
  Sang-Wook Kim (2)
  Yoon Seok Choi (1)
  Sea-Yuong Jeon (2)
  Vito Racanelli (3)
  Dae Woo Kim (4)
  Eui-Cheol Shin (1)
  
  1. Laboratory of Immunology and Infectious Diseases, Graduate School of Medical Science and Engineering, KAIST, Daejeon, 305-701, Republic of Korea
  2. Department of Otorhinolaryngology, Gyeongsang National University Hospital, Jinju, 660-702, Republic of Korea
  3. Department of Internal Medicine and Clinical Oncology, University of Bari Medical School, Bari, Italy
  4. Department of Otorhinolaryngology-Head and Neck Surgery, Boramae Medical Center, Seoul National University College of Medicine, Seoul, 156-707, Republic of Korea
  
• 刊物主题：Internal Medicine; Hematology; Oncology;
• 出版者：Springer Milan
• ISSN：1591-9528

文摘

The tonsils provide defense of the upper aerodigestive tract against pathogens. Although long known to undergo functional changes with age, the precise changes occurring within tonsillar B cell populations remain undefined. In the present study, we investigated age-related changes in palatine tonsillar B cell subsets and immunoglobulin (Ig) isotypes. Palatine tonsils were obtained from forty-two tonsillectomy patients without tonsillitis who were divided into three groups: young children (4–9 years), adolescents (10–19 years), and adults (20–60 years). Tonsillar B cells were then analyzed by flow cytometry. Using expression of CD38 and IgD to define B cell subsets, we found that the frequency of germinal center (GC) B cells in the tonsils was significantly higher, and the frequency of memory B cells lower, in young children as compared to adolescents and adults. Within the GC B cell subsets, adults had a higher frequency of IgA+ cells and a lower frequency of IgM+ cells as compared to individuals in the younger age groups. Moreover, young children had a higher frequency of IgG+ cells in the GC B cell subsets than did individuals in the older age groups. We also observed an abundance of IgM+ cells among memory B cells and plasmablasts in young children and IgA+ cells in adults. In summary, the proportion of GC B cells in palatine tonsillar B cells decreases with age, while the proportion of memory B cells increases with age. In addition, Ig isotypes in tonsils preferentially switch from IgM to IgA as individuals age. Keywords Age Tonsil B cell Subset Immunoglobulin Isotype