A transmembrane glycoprotein, gp38, is a novel marker for immature hepatic progenitor cells in fetal mouse livers

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• 作者：Sayuri Konishi (1)
  Kentaro Yasuchika (1)
  Takamichi Ishii (1) (2)
  Ken Fukumitsu (1)
  Naoko Kamo (1)
  Naoya Fujita (3)
  Iwao Ikai (1) (4)
  Shinji Uemoto (1)
  
• 关键词：Hepatic stem cell ; Hepatic progenitor cell ; gp38 ; Hepatocyte ; Wnt
• 刊名：In Vitro Cellular & Developmental Biology - Animal
• 出版年：2011
• 出版时间：January 2011
• 年：2011
• 卷：47
• 期：1
• 页码：45-53
• 全文大小：517KB
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• 作者单位：Sayuri Konishi (1)
  Kentaro Yasuchika (1)
  Takamichi Ishii (1) (2)
  Ken Fukumitsu (1)
  Naoko Kamo (1)
  Naoya Fujita (3)
  Iwao Ikai (1) (4)
  Shinji Uemoto (1)
  
  1. Department of Surgery, Graduate School of Medicine Kyoto University, 54 Kawahara-cho Shogoin,, Sakyo-ku,, Kyoto, 606-8507, Japan
  2. Laboratory of Embryonic Stem Cell Research, Stem Cell Research Center, Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan
  3. Cancer Chemotherapy Center, Japanese Foundation for Cancer Research, Tokyo, Japan
  4. Department of Surgery, Kyoto Medical Center, National Hospital Organization, Kyoto, Japan
  

文摘

Previously, we clarified the surface antigen profiles of hepatic progenitor cells (HPCs) in fetal liver tissue as the CD49f+CD45?/sup>Thy1?/sup> cell fraction. However, these cells were a heterogeneous cell population containing various stages of differentiation. This study aimed to detect more immature HPCs, using a novel surface antigen, gp38. After the collagenase digestion of fetal livers harvested from E13.5 to E18.5 fetal mice, HPCs were obtained and divided into two subpopulations using flow cytometry: gp38-positive HPCs, and gp38-negative HPCs. Both types of HPCs were characterized by immunocytochemistry and RT-PCR. The proliferative activity was compared by BrdU incorporation and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTS) assay. Furthermore, the comprehensive gene expression was investigated by DNA microarray. Both types of HPCs expressed alpha-fetoprotein. However, the gp38-positive HPCs derived from E13.5 fetal livers did not express albumin or cytokeratin 19, while the gp38-negative HPCs did. DNA microarray revealed that some genes related to the Wnt signal pathway were up-regulated in the gp38-positive HPCs. Furthermore, Wnt3a had a proliferative effect on the gp38-positive HPCs. In conclusion, the gp38-positive HPCs derived from fetal liver tissue until E13.5 could therefore be candidates for hepatic stem cells in the fetal liver.