Backbone and side-chain assignments of a tethered complex between LMO4 and DEAF-1
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- 作者：Soumya Joseph (1)
Ann H. Y. Kwan (1)
Joel P. Mackay (1)
Liza Cubeddu (2)
Jacqueline M. Matthews (1) - 关键词：LMO4 ; DEAF ; 1 ; Transcriptional complex ; Embryonic development ; Breast cancer
- 刊名：Biomolecular NMR Assignments
- 出版年：2014
- 出版时间：April 2014
- 年：2014
- 卷：8
- 期：1
- 页码：141-144
- 全文大小：437 KB
- 参考文献：1. Barker HE, Smyth GK, Wettenhall J, Ward TA, Bath ML, Lindeman GJ, Visvader JE (2008) Deaf-1 regulates epithelial cell proliferation and side-branching in the mammary gland. BMC Dev Biol 8:94 CrossRef
2. Cubeddu L, Joseph S, Richard DJ, Matthews JM (2012) Contribution of DEAF-1 structural domains to the interaction with the breast cancer oncogene LMO4. PLoS One 7(6):e39218 CrossRef
3. Hahm K, Sum EYM, Fujiwara Y, Lindeman GJ, Visvader JE, Orkin SH (2004) Defective neural tube closure and anteroposterior patterning in mice lacking the LIM protein LMO4 or its interacting partner Deaf-1. Mol Cell Biol 24(5):2074-082 CrossRef
4. Huggenvik JI, Michelson RJ, Collard MW, Ziemba AJ, Gurley P, Mowen KA (1998) Characterization of a nuclear deformed epidermal autoregulatory factor-1 (DEAF-1)-related (NUDR) transcriptional regulator protein. Mol Endocrinol 12(10):1619-639 CrossRef
5. Jensik PJ, Huggenvik JI, Collard MW (2004) Identification of a nuclear export signal and protein interaction domains in deformed epidermal autoregulatory factor-1 (DEAF-1). J Biol Chem 279(31):32692-2699 CrossRef
6. Kenny DA, Jurata LW, Saga Y, Gill GN (1998) Identification and characterization of LMO4, an LMO gene with a novel pattern of expression during embryogenesis. Proc Natl Acad Sci USA 95(19):11257-1262 CrossRef
7. Marley J, Lu M, Bracken C (2001) A method for efficient isotopic labeling of recombinant proteins. J Biomol NMR 20(1):71-5 CrossRef
8. Pelton JG, Torchia DA, Meadow ND, Roseman S (1993) Tautomeric states of the active-site histidines of phosphorylated and unphosphorylated IIIGlc, a signal-transducing protein from / Escherichia coli, using two-dimensional heteronuclear NMR techniques. Protein Sci 2(4):543-58 CrossRef
9. Shen Y, Delaglio F, Cornilescu G, Bax A (2009) TALOS+: a hybrid method for predicting protein backbone torsion angles from NMR chemical shifts. J Biomol NMR 44(4):213-23 CrossRef
10. Sugihara TM, Bach I, Kioussi C, Rosenfeld MG, Andersen B (1998) Mouse deformed epidermal autoregulatory factor 1 recruits a LIM domain factor, LMO-4, and CLIM coregulators. Proc Natl Acad Sci USA 95(26):15418-5423 CrossRef
11. Sum EYM, Segara D, Duscio B, Bath ML, Field AS, Sutherland RL, Lindeman GJ, Visvader JE (2005) Overexpression of LMO4 induces mammary hyperplasia, promotes cell invasion, and is a predictor of poor outcome in breast cancer. Proc Natl Acad Sci USA 102(21):7659-664 CrossRef
12. Yamazaki T, Forman-Kay JD, Kay LE (1993) Two-dimensional NMR experiments for correlating 13Cb and 1Hd/e chemical shifts of aromatic residues in 13C-labeled proteins via scalar couplings. J Am Chem Soc 115(23):11054-1055 CrossRef - 作者单位：Soumya Joseph (1)
Ann H. Y. Kwan (1)
Joel P. Mackay (1)
Liza Cubeddu (2)
Jacqueline M. Matthews (1)
1. School of Molecular Bioscience, University of Sydney, Sydney, NSW, 2006, Australia
2. School of Science and Health, University of Western Sydney, Sydney, NSW, 2751, Australia - ISSN：1874-270X
文摘
The transcriptional regulator LMO4 and the transcription factor DEAF-1 are both essential for brain and skeletal development. They are also implicated in human breast cancers; overexpression of LMO4 is an indicator of poor prognosis, and overexpression of DEAF-1 promotes epithelial breast cell proliferation. We have generated a stable LMO4–DEAF-1 complex comprising the C-terminal LIM domain of LMO4 and an intrinsically disordered LMO4-interaction domain from DEAF-1 tethered by a glycine/serine linker. Here we report the 1H, 15N and 13C assignments of this construct. Analysis of the assignments indicates the presence of structure in the DEAF-1 part of the complex supporting the presence of a physical interaction between the two proteins.