Microchip device for measurement of body volume of C. elegans as bioindicator application
详细信息 查看全文
- 作者：Jaehoon Jung (1)
Masahiro Nakajima (2)
Masaru Kojima (1)
Katsutoshi Ooe (1)
Toshio Fukuda (1) (2) - 关键词：C. elegans ; Bioindicator ; Microchip ; Tapered micro channel ; Capacitance change ; Cadmium
- 刊名：Journal of Micro-Nano Mechatronics
- 出版年：2012
- 出版时间：3 - December 2012
- 年：2012
- 卷：7
- 期：1
- 页码：3-11
- 全文大小：546KB
- 参考文献：1. Bao N, Wang J, Lu C (2008) Recent advances in electric analysis of cells in microfluidic systems. Anal Bioanal Chem 391(3):933鈥?42 CrossRef
2. Huang WH, Ai F, Wang ZL, Cheng JK (2008) Recent advances in single-cell analysis using capillary electrophoresis and microfluidic devices. J Chromatogr B 866(1鈥?):104鈥?22 CrossRef
3. Ahn CH, Choi JW, Beaucage G, Nevin JH, Lee JB, Puntambekar A, Lee JY (2004) Disposable smart lab on a chip for point-of-care clinical diagnostics. Proc IEEE 92(1):154鈥?73 CrossRef
4. Dittrich PS, Manz A (2006) Lab-on-a-chip: microfluidics in drug discovery. Nat Rev Drug Discov 5(3):210鈥?18 CrossRef
5. Kang L, Chung BG, Langer R, Khademhosseini A (2008) Microfluidics for drug discovery and development: from target selection to product lifecycle management. Drug Discov Today 13(1鈥?):1鈥?3 CrossRef
6. Roman GT, Kennedy RT (2007) Fully integrated microfluidic separations systems for biochemical analysis. J Chromatogr A 1168(1鈥?):170鈥?88
7. Chronis N (2009) Worm chips: microtools for / C. elegans biology. Lab Chip 10(4):432鈥?37 CrossRef
8. Fakhoury JR, Sisson JC, Zhang X (2009) Microsystems for controlled genetic perturbation of live / Drosophila embryos: RNA interference, development robustness and drug screening. Microfluid Nanofluidics 6(3):299鈥?13 CrossRef
9. Johnson TE (2008) / Caenorhabditis elegans 2007: the premier model for the study of aging. Exp Gerontol 43(1):1鈥?
10. Ben-Yakar A, Chronis N, Lu H (2009) Microfluidics for the analysis of behavior, nerve regeneration, and neural cell biology in / C. elegans. Curr Opin Neurobiol 19(5):561鈥?67 CrossRef
11. Ma H, Jiang L, Shi W, Qin J, Lin B (2009) A programmable microvalve-based microfluidic array for characterization of neurotoxin-induced responses of individual / C. elegans. Biomicrofluidics 3:044114 CrossRef
12. Leung MCK, Williams PL, Benedetto A, Au C, Helmcke KJ, Aschner M, Meyer JN (2008) / Caenorhabditis elegans: an emerging model in biomedical and environmental toxicology. Toxicol Sci 106(1):5 CrossRef
13. Wah Chu K, Chow KL (2002) Synergistic toxicity of multiple heavy metals is revealed by a biological assay using a nematode and its transgenic derivative. Aquat Toxicol 61(1鈥?):53鈥?4 CrossRef
14. Swain S, Keusekotten K, Baumeister R, Sturzenbaum S (2004) C. elegans metallothioneins: new insights into the phenotypic effects of cadmium toxicosis. J Mol Biol 341(4):951鈥?59 CrossRef
15. Roh JY, Lee J, Choi J (2006) Assessment of stress related gene expression in the heavy metal exposed nematode / Caenorhabditis elegans: a potential biomarker for metal induced toxicity monitoring and environmental risk assessment. Environ Toxicol Chem 25(11):2946鈥?956 CrossRef
16. Wang D, Xing X (2008) Assessment of locomotion behavioral defects induced by acute toxicity from heavy metal exposure in nematode / Caenorhabditis elegans. J Environ Sci 20(9):1132鈥?137 CrossRef
17. Hope I Background on / Caenorhabditis elegans. C elegans a practical approach:1鈥?5
18. Hall DH, Altun ZF (2008) / C. elegans atlas. Cold Spring Harbor Laboratory Pr.
19. Shi W, Wen H, Lu Y, Shi Y, Lin B, Qin J (2010) Droplet microfluidics for characterizing the neurotoxin-induced responses in individual / Caenorhabditis elegans. Lab Chip 10(21):2855鈥?863 CrossRef
20. Rohde CB, Zeng F, Gonzalez-Rubio R, Angel M, Yanik MF (2007) Microfluidic system for on-chip high-throughput whole-animal sorting and screening at subcellular resolution. Proc Natl Acad Sci 104(35):13891 CrossRef
21. Qin J, Wheeler AR (2006) Maze exploration and learning in / C. elegans. Lab Chip 7(2):186鈥?92 CrossRef
22. Chung K, Crane MM, Lu H (2008) Automated on-chip rapid microscopy, phenotyping and sorting of / C. elegans. Nat Methods 5(7):637鈥?43 CrossRef
23. Chronis N, Zimmer M, Bargmann CI (2007) Microfluidics for in vivo imaging of neuronal and behavioral activity in / Caenorhabditis elegans. Nat Methods 4(9):727鈥?31 CrossRef
24. Hulme SE, Shevkoplyas SS, McGuigan AP, Apfeld J, Fontana W, Whitesides GM (2010) Lifespan-on-a-chip: microfluidic chambers for performing lifelong observation of / C. elegans. Lab Chip 10(5):589鈥?97 CrossRef
25. Krajniak J, Lu H (2010) Long-term high-resolution imaging and culture of / C. elegans in chip-gel hybrid microfluidic device for developmental studies. Lab Chip 10(14):1862鈥?868 CrossRef
26. Hulme SE, Shevkoplyas SS, Apfeld J, Fontana W, Whitesides GM (2007) A microfabricated array of clamps for immobilizing and imaging / C. elegans. Lab Chip 7(11):1515鈥?523 CrossRef
27. Ferrier G, Hladio A, Thomson D, Bridges G, Hedayatipoor M, Olson S, Freeman M (2008) Microfluidic electromanipulation with capacitive detection for the mechanical analysis of cells. Biomicrofluidics 2:044102 CrossRef
28. Gawad S, Schild L, Renaud P (2001) Micromachined impedance spectroscopy flow cytometer for cell analysis and particle sizing. Lab Chip 1 (1):76-82 CrossRef
29. Morgan H, Sun T, Holmes D, Gawad S, Green NG (2007) Single cell dielectric spectroscopy. Journal of Physics D: Appl Phys 40:61
30. Rezai P, Siddiqui A, Selvaganapathy PR, Gupta BP (2009) Electrotaxis of / Caenorhabditis elegans in a microfluidic environment. Lab Chip 10(2):220鈥?26 CrossRef
31. Chuang HS, Raizen DM, Lamb A, Dabbish N, Bau HH (2011) Dielectrophoresis of / Caenorhabditis elegans. Lab Chip
32. Popham J, Webster J (1979) Cadmium toxicity in the free-living nematode, Caenorhabditis elegans. Environ Res 20(1):183鈥?91 CrossRef
33. Williams PL, Dusenbery DB (1990) Aquatic toxicity testing using the nematode, / Caenorhabditis elegans. Environ Toxicol Chem 9(10):1285鈥?290
34. Whitesides GM, Ostuni E, Takayama S, Jiang X, Ingber DE (2001) Soft lithography in biology and biochemistry. Annu Rev Biomed Eng 3(1):335鈥?73 CrossRef
35. Brenner S (1974) The genetics of Caenorhabditis elegans. Genetics 77(1):71
36. Byerly L, Cassada R, Russell R (1976) The life cycle of the nematode / Caenorhabditis elegans 1, I. Wild-type growth and reproduction. Dev Biol 51(1):23鈥?3 CrossRef
37. Collins JJ, Huang C, Hughes S, Kornfeld K (2008) The measurement and analysis of age-related changes in Caenorhabditis elegans. WormBook 24:1鈥?1
38. Strange K (2006) C. elegans: methods and applications, vol 351. Humana Pr Inc,
39. Byerly L, Cassada R, Russell R (1975) Machine for rapidly counting and measuring the size of small nematodes. Rev Sci Instrum 46(5):517鈥?22 CrossRef
40. Page AP, Johnstone I (2007) The cuticle. WormBook - 作者单位：Jaehoon Jung (1)
Masahiro Nakajima (2)
Masaru Kojima (1)
Katsutoshi Ooe (1)
Toshio Fukuda (1) (2)
1. Department of Micro-Nano Systems Engineering, Nagoya University, Nagoya, Japan
2. Center for Micro-nano Mechatronics, Nagoya University, Nagoya, Japan - ISSN：1865-3936
文摘
We propose a micro fluidic device to analyze Caenorhabditis elegans (C. elegans) as a bioindicator. In this device, we assessed the body volume of C. elegans using a microchip with a capacitance sensor. This device is composed of two key parts. One is a tapered micro channel. The tapered micro channel is used to immobilize C. elegans. The other is a pair of electrodes. The electrodes are used to measure the capacitance change. These two components form a sensing space; a capacitance change occurs when C. elegans is placed in the sensing space. In this study, we performed two kinds of experiments. First, to check the correlation between the body volume and the capacitance change, a total of 15 C. elegans were measured; from L2 larval stage to adult stage. Second, to confirm the possibility of the proposed microchip as a device that can analyze the condition of C. elegans as a bioindicator, a total of 35 C. elegans were measured. To measure the body volume as a reference, first, the body length and the diameter of C. elegans were measured by an optical microscope and then the volume was calculated from those two measured values. The capacitance change was measured by the proposed microchip. By comparison between the result of two methods, we confirmed the correlation between the calculated values. The possibility of the proposed microchip as the device to analyze the effect of cadmium on C. elegans as a bioindicator was established.