Genetic diversity of a Korean echovirus 5 isolate and response of the strain to five antiviral drugs

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• 作者：Kwisung Park (1)
  Jaehyoung Song (2)
  Kyoungah Baek (1)
  Changgyun Lee (1)
  Donguk Kim (1)
  SamHyun Cho (3)
  JoonSoo Park (4)
  YoungJin Choi (5)
  Byunghak Kang (6)
  Hwajung Choi (7)
  Doo-Sung Cheon (6)
  
• 刊名：Virology Journal
• 出版年：2011
• 出版时间：December 2011
• 年：2011
• 卷：8
• 期：1
• 全文大小：400KB
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• 作者单位：Kwisung Park (1)
  Jaehyoung Song (2)
  Kyoungah Baek (1)
  Changgyun Lee (1)
  Donguk Kim (1)
  SamHyun Cho (3)
  JoonSoo Park (4)
  YoungJin Choi (5)
  Byunghak Kang (6)
  Hwajung Choi (7)
  Doo-Sung Cheon (6)
  
  1. Department of Microbiology, Chungcheongnam-Do Institute of Health and Environmental Research, Daejeon, Korea
  2. Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University, Iksan, Korea
  3. Department of Obstetrics and Gynecology, College of Medicine, Hanyang University, Seoul, Korea
  4. Department of Pediatrics, College of Medicine, Soonchunhyang University, Cheonan, Korea
  5. Department of Laboratory Medicine, College of Medicine, Soonchunhyang University, Cheonan, Korea
  6. Department of Enteric and Hepatitis Viruses, National Institute of Health, Korea Center for Disease Control and Prevention, Seoul, Korea
  7. Department of Clinical Pathology, Daejeon Health Science College, Daejeon, Korea
  
• ISSN：1743-422X

文摘

An outbreak of echovirus 5 (ECV 5) occurred in Korea in 2006, marking the first time this virus had been identified in the country since enterovirus surveillance began in 1993. Using a sample isolated from a young male patient with aseptic meningitis, we performed sequencing of the Korean ECV 5 strain and compared it with a prototype strain (Noyce). At the nucleotide level, the P1 region (85.3%) had the highest identity value; at the amino acid level, the P3 region (98.0%) had the highest identity value. The two strains shared all cleavage sites, with the exception of the VP1/2A site, which was TY/GA in the Noyce strain but TR/GA in the Korean ECV 5 isolate. In Vero cells infected with the Korean ECV 5 isolate, no cytotoxicity was observed in the presence of azidothymidine, acyclovir, amantadine, lamivudine, or ribavirin, when the drugs were administered at a CC50 value >100 μg/mL. Of the five drugs, only amantadine (IC50: 1 ± 0.42 μg/mL, TI: 100) and ribavirin (IC50: 22 ± 1.36 μg/mL, TI: 4.55) had any antiviral activity against the Korean ECV 5 isolate.